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A plant-based extract mixture for controlling Spodoptera litura (Lepidoptera: Noctuidae)
Chemical and Biological Technologies in Agriculture ( IF 5.2 ) Pub Date : 2019-03-14 , DOI: 10.1186/s40538-019-0143-6
Thitaree Yooboon , Anchulee Pengsook , Atcharee Ratwatthananon , Wanchai Pluempanupat , Vasakorn Bullangpoti

Spodoptera litura larvae are polyphagous insects that have become a significant pest in recent years. The spread of this pest has led to the continuous usage of insecticides on crops. Some plant extracts have been used as a mixture to control insect pests and improve productivity. A plant-based mixture was mixed at a ratio of 1:1 v/v to demonstrate the effect on contact toxicity, feeding (no-choice test), and enzyme activities on S. litura. The active compounds of P. retrofractum and A. calamus were isolated by preparative thin-layer chromatography (PTLC). Our results showed that binary mixtures from P. retrofractum and A. calamus exhibit the highest contact toxicity and antifeedant activity at a 1:1 ratio of LD30:LD10 dose (3.213 µg/larva P. retrofractum + 3.294 µg/larva A. calamus). The main active ingredient from each crude extract was (2E,4E,14Z)-N-isobutylicosa-2,4,14-trienamide from P. retrofractum, and beta-asarone and alpha-asarone from A. calamus. Additionally, A. calamus seems to be the synergistic compound. Some compound mixtures increased the glutathione-S-transferase activities in vivo; whereas, almost no significant differences in esterase activities were noted. The results indicated that the ethanolic crude extracts of P. retrofractum and A. calamus mixtures could be used as the pesticidal compound and to develop a binary mixture formulation for controlling lepidopteran pests. However, the toxicity of this mixture to mammals needed to be explored before commercial development.

中文翻译:

基于植物的提取物混合物,用于控制斜纹夜蛾(鳞翅目:夜蛾科)

斜纹夜蛾幼虫是多食性昆虫,近年来已成为重要的害虫。这种有害生物的传播导致在农作物上不断使用杀虫剂。一些植物提取物已被用作控制害虫和提高生产力的混合物。以1:1 v / v的比例混合植物基混合物,以证明其对接触毒性,进食(无选择试验)和酶对斜纹夜蛾的活性的影响。通过制备薄层色谱法(PTLC)分离出逆向体育和。蒲的活性化合物。我们的结果表明,来自逆向体育杆菌和and蒲的二元混合物在LD30:LD10剂量为1:1的比例下(3.213 µg /幼虫逆向分数线虫3.329 µg /幼体A蒲)显示出最高的接触毒性和拒食活性。 。每种粗提物中的主要活性成分为(2E,4E,逆向体育杆菌(P.retrofractum)的14Z)-N-异丁基icosa-2,4,14-三烯酰胺,以及cal蒲(A. calamus)的β-asarone和α-asarone。此外,cal蒲似乎是协同化合物。一些化合物混合物增加了体内谷胱甘肽-S-转移酶的活性。酯酶活性几乎没有显着差异。结果表明,乙醇提取物P. retrofractum和A. calamus的混合物可以用作农药化合物,并开发出防治鳞翅目害虫的二元混合物配方。然而,这种混合物对哺乳动物的毒性需要在商业开发之前进行探索。此外,cal蒲似乎是协同化合物。一些化合物混合物增加了体内谷胱甘肽-S-转移酶的活性。酯酶活性几乎没有显着差异。结果表明,乙醇提取物P. retrofractum和A. calamus的混合物可以用作农药化合物,并开发出防治鳞翅目害虫的二元混合物配方。然而,这种混合物对哺乳动物的毒性需要在商业开发之前进行探索。此外,cal蒲似乎是协同化合物。一些化合物混合物增加了体内谷胱甘肽-S-转移酶的活性。酯酶活性几乎没有显着差异。结果表明,乙醇提取物P. retrofractum和A. calamus的混合物可以用作农药化合物,并开发出防治鳞翅目害虫的二元混合物配方。然而,这种混合物对哺乳动物的毒性需要在商业开发之前进行探索。结果表明,乙醇提取物P. retrofractum和A. calamus的混合物可以用作农药化合物,并开发出防治鳞翅目害虫的二元混合物配方。然而,这种混合物对哺乳动物的毒性需要在商业开发之前进行探索。结果表明,乙醇提取物P. retrofractum和A. calamus的混合物可以用作农药化合物,并开发出防治鳞翅目害虫的二元混合物配方。然而,这种混合物对哺乳动物的毒性需要在商业开发之前进行探索。
更新日期:2019-03-14
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