N fertilizers make the total cost of production of biofuels, sugar and bioelectricity expensive in the sugar-energy industry. Microbes could replace N fertilizers in the nutritional management of sugarcane crop by capturing N₂ from the atmosphere. We accordingly conducted this scientific study to investigate the response of sugarcane crop to Azospirillum brasilense. We tested the strain Az-V5 at the doses of 0, 50, 100, 200 and 400 mL ha⁻¹, by spraying it into the planting furrow, 7, 14 and 21 days after crop planting into 20-l pots filled with sieved Rhodic Hapludox, with a loamy texture. In order to examine biologically modulated morphological changes in the host plant material, namely sugarcane variety ‘RB86-7515,’ we harvested and then assessed 90-day-old plants for plant height, stalk diameter, number of tillers, number of leaves, leaf area, tiller length, dry matter of shoots and root dry matter. Sugarcane plants treated with the bioagent 14 and 21 days after crop planting had lower mean values of plant height, number of leaves, leaf area, dry matter of shoots and root dry matter. Therefore, applying A. brasilense into the planting furrow, instead of spraying it on sugarcane leaves, could protect it for a longer period from biotic and abiotic factors influencing cell viability, root colonization and ultimately symbiosis. The conclusion is, therefore, application of A. brasilense into planting furrow is the best environmentally friendly and low-cost strategy to promote growth and development in sugarcane plants at the beginning of their life cycle.

中文翻译：

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促进植物生长的细菌巴西细螺旋藻诱导甘蔗作物的形态变化

氮肥使糖能源行业的生物燃料，糖和生物电的生产总成本昂贵。在甘蔗作物的营养管理中，微生物可以通过从大气中捕获N ₂来替代N肥料。因此我们进行了这项科学研究，以研究甘蔗作物对巴西拟螺旋体的反应。我们以0、50、100、200和400 mL ha ^-1的剂量测试了Az-V5菌株，在农作物播种后的7、14、21天，将其喷洒到种植沟中，注入装有肥沃的筛分的Rhodic Hapludox的20升花盆中，肥沃的质地。为了检查宿主植物材料（即甘蔗品种“ RB86-7515”）中的生物调控形态变化，我们收获了然后评估了90天龄的植物的株高，茎直径，分ers数，叶数，叶数面积，分till长度，芽的干物质和根干物质。作物种植后第14天和第21天，用生物制剂处理的甘蔗植株的平均高度，叶片数，叶面积，枝干和根干物质的平均值较低。因此，应用巴西青霉进入种植沟，而不是将其喷在甘蔗叶上，可以在更长的时间内保护它免受影响细胞活力，根部定植并最终共生的生物和非生物因素的影响。因此，结论是，将巴西拟南芥用于种植犁沟是在甘蔗植物生命周期开始时促进其生长发育的最佳环保和低成本策略。