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The complete life cycle of the unusual apostome Hyalophysa clampi (Ciliophora, Apostomatida), a symbiont of crayfish in Alabama (USA)
European Journal of Protistology ( IF 2.9 ) Pub Date : 2019-11-08 , DOI: 10.1016/j.ejop.2019.125654
Randall D Bassham 1 , Jeremy S Browning 2 , Stephen C Landers 1
Affiliation  

Hyalophysa clampi Browning and Landers, 2012 was reexamined to determine all stages in the life cycle of this symbiotic ciliate. The cell feeds as a normal exuviotroph within the exoskeleton of its molted crayfish host but does not encyst following the trophont stage. Trophonts transform into swimming tomont stages, which divide by palintomy over successive divisions, splitting to two cells, separating, and repeating. The divisions cease when the daughter cells attain the size of the infestive tomite stage, which attaches to a new crayfish. This unique life cycle is most similar to the European hermit crab symbiont Polyspira delagei, which forms chains of daughter cells during division. Scanning electron microscopy confirmed the unusual presence of two contractile vacuoles in H. clampi, unique among the Apostomatida, and provided ultrastructural details to better understand light microscopy silver staining. The genus diagnosis for Hyalophysa is modified herein to accommodate this new life cycle.



中文翻译:

在阿拉巴马州(美国),小龙虾的共生体,不寻常的使者Hyalophysalampi(Ciliophora,Apostomatida)的完整生命周期。

HyalophysalampiBrowning和Landers,2012年进行了重新检查,以确定这种共生纤毛虫生命周期的所有阶段。该细胞在其蜕皮的小龙虾宿主的外骨骼中作为正常的exophiotroph摄食,但在风化阶段后不会包囊。Trophonts转变为游泳的tomont阶段,该阶段通过回旋术分成连续的多个部分,分裂成两个单元格,分离并重复。当子细胞达到侵袭性m突阶段的大小时,分裂停止,该阶段附加到新的小龙虾上。这种独特的生命周期最类似于欧洲寄居蟹共生菌Polyspira delagei,后者在分裂过程中形成子细胞链。扫描电子显微镜证实,在体内异常存在两个收缩液泡H.lampi,在特异科中独特,并提供了超微结构细节,以更好地了解光学显微镜的银染。Hyalophysa的属诊断在此进行了修改,以适应这种新的生命周期。

更新日期:2019-11-08
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