This study investigated the characterization and functionality of Undaria pinnatifida root (UPT) extracts, degraded using a crude enzyme from Shewanella oneidensis PKA1008. To obtain the optimum degrading conditions, the UPT was mixed with alginate degrading enzymes from S. oneidensis PKA 1008 and was incubated at 30°C for 0, 3, 6, 12, 24, and 48 h. The alginate degrading ability of these enzymes was then evaluated by measuring the reducing sugar, viscosity, pH and chromaticity. Enzymatic extract at 24 h revealed the highest alginate degrading ability and the lowest pH value. As the incubation time increased, the lightness (L *) also decreased and was measured at its lowest value, 39.84, at 12 hours. The redness and yellowness increased gradually to 10.27 at 6 h and to 63.95 at 3 h, respectively. Moreover, the alginate oligosaccharides exhibited significant anti-inflammatory activity. These results indicate that a crude enzyme from S. oneidensis PKA 1008 can be used to enhance the polysaccharide degradation of UPT and the alginate oligosaccharides may also enhance the anti-inflammatory effect.

中文翻译：

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使用希瓦氏菌 PKA 1008 粗酶对裙带菜根酶提取物进行表征及其抗炎作用。


本研究调查了裙带菜根 (UPT) 提取物的特性和功能，该提取物使用来自Shewanella oneidensis PKA1008 的粗酶进行降解。为了获得最佳降解条件，将 UPT 与来自S. oneidensis PKA 1008 的藻酸盐降解酶混合，并在 30°C 下孵育 0、3、6、12、24 和 48 小时。然后通过测量还原糖、粘度、pH 值和色度来评估这些酶的藻酸盐降解能力。 24 h 时的酶提取物显示出最高的藻酸盐降解能力和最低的 pH 值。随着孵育时间的增加，亮度 (L *) 也降低，并在 12 小时时测得其最低值 39.84。红度和黄度分别逐渐增加到 6 小时时的 10.27 和 3 小时时的 63.95。此外，藻酸盐寡糖表现出显着的抗炎活性。这些结果表明，来自S. oneidensis PKA 1008的粗酶可用于增强UPT的多糖降解，并且藻酸盐寡糖也可增强抗炎作用。