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Potential antibiotic-producing fungal strains isolated from pharmaceutical waste sludge
Beni-Suef University Journal of Basic and Applied Sciences ( IF 2.5 ) Pub Date : 2019-12-01 , DOI: 10.1186/s43088-019-0026-8
Sunday Osaizua Omeike , Sarafadeen Olateju Kareem , Adebayo Aliyu Lasisi

Antibiotic resistance and dearth of novel compounds from natural sources warrants the need to search other environments for potential antibiotic-producing microbial species. The study investigated isolation and identification of antibiotic-producing fungi from pharmaceutical waste sludge. Seven hundred and ninety-seven isolates obtained from sludge of seven pharmaceutical industries in Sango Ota, Ogun State using several growth media, with mould isolates highest (696). Isolated species were from genera Aspergillus (28.55%), Penicillium (18.35%), Trichoderma (13.44%), Rhizopus (10.21%) and Geotrichum (4.01%), and Stachybotrys (0.13%). The CFS of strains named Geotrichum candidum OMON-1, Talaromyces pinophilus OKHAIN-12, and Penicillium citrinum PETER-OOA1 had high reproducible bioactivity against Staphylococcus aureus (32 ± 0.12 mm) and Klebsiella pneumoniae (29 ± 0.12 mm) while P. citrinum MASTER-RAA2 had activity against K. pneumoniae only. Active metabolites were successfully extracted using Diaion HP-20 and methanol:iso-propanol:acetone (6:3:1 v/v). Antibacterial-active fractions of fungal extract successfully eluted with 40–60% NaCl on ion-exchange chromatography using a cation column. The study successfully screened antibiotic-producing fungal species from pharmaceutical waste storage facilities. Study also showed that similar species from same toxic environment could potentially produce different metabolites.

中文翻译:

从制药废污泥中分离出潜在的抗生素生产真菌菌株

抗生素耐药性和来自天然来源的新型化合物的缺乏需要在其他环境中寻找潜在的抗生素产生微生物物种。该研究调查了从制药废污泥中分离和鉴定产生抗生素的真菌。使用几种生长培养基从奥贡州 Sango Ota 七个制药工业的污泥中获得 797 个分离株,其中霉菌分离株最高 (696)。分离的物种来自曲霉属 (28.55%)、青霉属 (18.35%)、木霉属 (13.44%)、根霉属 (10.21%) 和地霉属 (4.01%) 和葡萄孢属 (0.13%)。名为 Geotrichum candidum OMON-1、Talaromyces pinophilus OKHAIN-12 和 Penicillium citrinum PETER-OOA1 的菌株的 CFS 对金黄色葡萄球菌具有高重现性的生物活性 (32 ± 0. 12 毫米)和肺炎克雷伯菌(29 ± 0.12 毫米),而柑橘假单胞菌 MASTER-RAA2 仅对肺炎克雷伯菌有活性。使用 Diaion HP-20 和甲醇:异丙醇:丙酮 (6:3:1 v/v) 成功提取了活性代谢物。在使用阳离子柱的离子交换色谱上,真菌提取物的抗菌活性部分成功地用 40-60% NaCl 洗脱。该研究成功地从制药废物储存设施中筛选了产生抗生素的真菌物种。研究还表明,来自相同有毒环境的相似物种可能会产生不同的代谢物。在使用阳离子柱的离子交换色谱上,真菌提取物的抗菌活性部分用 40-60% NaCl 成功洗脱。该研究成功地从制药废物储存设施中筛选了产生抗生素的真菌物种。研究还表明,来自相同有毒环境的相似物种可能会产生不同的代谢物。在使用阳离子柱的离子交换色谱上,真菌提取物的抗菌活性部分成功地用 40-60% NaCl 洗脱。该研究成功地从制药废物储存设施中筛选了产生抗生素的真菌物种。研究还表明,来自相同有毒环境的相似物种可能会产生不同的代谢物。
更新日期:2019-12-01
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