Accurate determination of sex ratios is an important metric for the conservation and management of wild Atlantic salmon populations. Previously published primers for a male-specific gene were shown to be unreliable at determining sex due to the presence of deletions in the forward primer-binding site. A new forward primer, used in conjunction with the existing reverse primer, greatly improved reliability of the genetic test, correctly assigning gender in samples of known sex. Using DNA extracted from both contemporary and archive tissues, comparison between the two primer sets screened on collections of unknown sex ratio showed significant discrepancies in the number of males identified. The new primer will help improve the management and conservation of Atlantic salmon by providing accurate sex ratios for the setting of Conservation Limits.

准确确定性别比是保护和管理野生大西洋鲑鱼种群的重要指标。由于正向引物结合位点中存在缺失，先前公开的男性特异性基因引物显示出在确定性别方面不可靠。一种新的正向引物，与现有的反向引物结合使用，大大提高了基因测试的可靠性，在已知性别的样本中正确分配了性别。使用从当代和档案馆组织中提取的DNA，在性别比例未知的集合中筛选的两个引物组之间的比较显示，鉴定出的男性数量存在显着差异。新底漆将通过提供精确的性别比来设定保护限量，帮助改善大西洋鲑的管理和保护。