Chemical modification of titanium/titanium oxide (Ti/TiO₂) substrates has recently gained a great deal of attention because of the applications of Ti/TiO₂-based materials to biomedical areas. The reported modification methods generally involve passive coating of Ti/TiO₂ substrates with protein-resistant materials, and poly(ethylene glycol) (PEG) has proven advantageous for bestowing a nonbiofouling property on the surface of Ti/TiO₂. However, the wider applications of Ti/TiO₂-based materials to biomedical areas will require the introduction of biologically active moieties onto Ti/TiO₂, in addition to nonbiofouling property. In this work, we therefore utilized surface-initiated polymerization to coat the Ti/TiO₂ substrates with polymers presenting the nonbiofouling PEG moiety and subsequently conjugated biologically active compounds to the PEG-presenting, polymeric films. Specifically, a Ti/TiO₂ surface was chemically modified to present an initiator for atom transfer radical polymerization, and poly(ethylene glycol) methacrylate (PEGMA) was polymerized from the surface. After activation of hydroxyl groups of poly(PEGMA) (pPEGMA) withN,N′-disuccinimidyl carbonate, biotin, a model compound, was conjugated to the pPEGMA films. The reactions were confirmed by infrared spectroscopy, X-ray photoelectron spectroscopy, contact angle goniometry, and ellipsometry. The biospecific binding of target proteins was also utilized to generate micropatterns of proteins on the Ti/TiO₂ surface.

中文翻译：

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链霉亲和素通过表面引发的原子转移自由基聚合和生物素的生物缀合与非生物污染的钛/二氧化钛表面特异性结合

由于基于Ti / TiO ₂的材料在生物医学领域的应用，最近钛/二氧化钛（Ti / TiO ₂）基材的化学改性引起了广泛的关注。所报道的改性方法通常包括用抗蛋白质材料对Ti / TiO ₂基材进行被动涂覆，并且聚乙二醇（PEG）已被证明有利于赋予Ti / TiO ₂表面非生物污垢特性。但是，基于Ti / TiO ₂的材料在生物医学领域的广泛应用将需要在Ti / TiO ₂上引入生物活性部分。，以及非生物污染特性。因此，在这项工作中，我们利用表面引发的聚合反应在Ti / TiO ₂基材上涂覆了呈现非生物结垢PEG部分的聚合物，并随后将生物活性化合物偶联到了PEG呈现的聚合物薄膜上。具体地，对Ti / TiO ₂表面进行化学修饰以提供用于原子转移自由基聚合的引发剂，并且从该表面聚合聚（甲基丙烯酸乙二醇酯）（PEGMA）。用N，N活化聚（PEGMA）（pPEGMA）的羟基后碳酸二琥珀酰亚胺酯，一种生物素，一种模型化合物，与pPEGMA膜结合。通过红外光谱，X射线光电子能谱，接触角测角法和椭偏法确认了反应。靶蛋白的生物特异性结合也用于在Ti / TiO ₂表面上产生蛋白的微图案。