Non-typhoidal Salmonella (NTS) are a significant source of foodborne illness worldwide, with disease symptoms most often presenting as self-limiting gastroenteritis; however, occasionally the infection spreads and becomes invasive, frequently requiring anti-microbial treatment. The cattle-adapted Dublin serovar of NTS has commonly been associated with invasive illness and anti-microbial resistance (AMR). Here, the enhanced resolution conferred by whole-genome sequencing was utilized to elucidate and compare the resistome and genetic relatedness of 14 multidrug-resistant (MDR) and one pan-susceptible S. Dublin, isolated primarily in Pennsylvania, from fresh retail meat (one isolate) and humans (14 isolates). Twelve different genetic AMR determinants, including both acquired and chromosomal, were identified. Furthermore, comparative plasmid analysis indicated that AMR was primarily conferred by a putative IncA/C2 plasmid. A single pan-susceptible S. Dublin isolate, collected from the same timeframe and geographical region as the MDR isolates, did not carry an IncA/C2 replicon sequence within its genome. Moreover, the pan-susceptible isolate was genetically distinct from its MDR counterparts, as it was separated by ≥267 single nucleotide polymorphisms (SNPs), whereas there was a ≤38 SNP distance between the MDR isolates. Collectively, this data set advances our understanding of the genetic basis of the highly drug-resistant nature of S. Dublin, a serovar with significant public health implications.

中文翻译：

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全基因组测序揭示了高度抗药性零售肉和人沙门氏菌都柏林的抗药性。

非伤寒沙门氏菌（NTS）是全世界食源性疾病的重要来源，其疾病症状最常表现为自限性肠胃炎。但是，有时感染会扩散并变成侵入性的，经常需要进行抗微生物治疗。牛适应的NTS都柏林血清型通常与侵袭性疾病和抗药性（AMR）相关。在这里，通过全基因组测序赋予的增强分辨率来阐明和比较从新鲜零售肉类中分离的14种多药抗药性（MDR）和一种主要在宾夕法尼亚州分离的易感性都柏林链霉菌（S. Dublin）的耐药性和遗传相关性（一种分离株）和人类（14个分离株）。鉴定了十二种不同的遗传AMR决定因素，包括后天和染色体。此外，对比质粒分析表明，AMR主要由推定的IncA / C2质粒赋予。从与MDR分离株相同的时间范围和地理区域收集的单一易感性都柏林链球菌都没有在其基因组中携带IncA / C2复制子序列。此外，全敏感性分离株与MDR对应株在遗传上是不同的，因为它被≥267个单核苷酸多态性（SNP）隔开，而MDR分离株之间的距离小于或等于38。总的来说，这些数据集使我们对S. Dublin（一种具有重大公共卫生意义的血清型）的高度耐药性的遗传基础有了更深入的了解。从与MDR分离物相同的时间范围和地理区域收集的，其基因组中未携带IncA / C2复制子序列。此外，全敏感性分离株与MDR对应株在遗传上是不同的，因为它被≥267个单核苷酸多态性（SNP）隔开，而MDR分离株之间的距离小于或等于38。总的来说，这些数据集使我们对S. Dublin（一种具有重大公共卫生意义的血清型）的高度耐药性的遗传基础有了更深入的了解。从与MDR分离物相同的时间范围和地理区域收集的，其基因组中未携带IncA / C2复制子序列。此外，全敏感性分离株与MDR对应株在遗传上是不同的，因为它被≥267个单核苷酸多态性（SNP）隔开，而MDR分离株之间的距离小于或等于38。总的来说，这些数据集使我们对S. Dublin（一种具有重大公共卫生意义的血清型）的高度耐药性的遗传基础有了更深入的了解。