In this study, field and laboratory experiments were carried out in order to develop a biological control strategy against mustard aphid by using extracts of five tobacco varieties (Beeri wala, Balkhi, Pattay wala, Desi Tobacco and Hazropattar). Two extraction methods were used: extraction through boiling of leaves (ETBL) and extraction through overnight water soaking of leaves (ETOWSL). Mustard variety (Early Mustard) was cultivated with eleven treatments of three replicates each. Laboratory experiments were conducted to confirm the mortality due to the extract of tobacco varieties and the efficient type of extraction. All treatments were found to be effective in field against mustard aphid. Extracts of Pattay wala, Balkhi and Desi tobacco obtained through ETBL remained effective up to a week, whereas the extracts of Hazropattar, Balkhi, Pattay wala and Desi tobacco obtained through ETOWSL were found to be effective up to two weeks after treatment. The highest reduction in aphid population was noted by Pattay wala tobacco and the lowest reduction was seen by Desi tobacco. Almost the same trend of effectiveness was recorded during 2nd spray. In laboratory conditions 100% mortality of aphids was observed within three days under all treatments. The results of this study could be useful in an integrated pest management program for the management of mustard aphid Lipaphis erysimi.

中文翻译：

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不同烟草品种的叶提取物对芥菜蚜虫的功效比较（Lipaphis erysimi Kalt。）

在这项研究中，进行了野外和实验室实验，以通过使用五个烟草品种（Beeri wala，Balkhi，Pattay wala，Desi Tobacco和Hazropattar）的提取物来开发针对芥菜蚜的生物防治策略。使用了两种提取方法：通过煮沸叶提取（ETBL）和通过过夜水浸泡叶提取（ETOWSL）。芥菜品种（Early Mustard）通过11种处理方法进行栽培，每种处理重复3次。进行了实验室实验，以确认由于烟草品种的提取物引起的死亡率和提取物的有效类型。发现所有处理在田间均有效对抗芥菜蚜虫。通过ETBL获得的Pattay wala，Balkhi和Desi烟草的提取物有效期长达一周，而Hazropattar，Balkhi，发现通过ETOWSL获得的Pattay wala和Desi烟草在治疗后两周内有效。Pattay wala烟草的蚜虫种群减少最高，而Desi烟草的蚜虫种群减少最少。在第二次喷洒期间记录到几乎相同的效果趋势。在实验室条件下，所有处理均在三天内观察到了100％的蚜虫死亡率。这项研究的结果可能对芥菜蚜虫的综合害虫管理计划有用 在实验室条件下，所有处理均在三天内观察到了100％的蚜虫死亡率。这项研究的结果可能对芥菜蚜虫的综合害虫管理计划有用 在实验室条件下，所有处理均在三天内观察到了100％的蚜虫死亡率。这项研究的结果可能对芥菜蚜虫的综合害虫管理计划有用 Lipaphis erysimi。