To assist in efforts to monitor the distribution and abundance of lake sturgeon Acipenser fulvescens, a species of conservation concern in Canada and the United States, we developed two quantitative PCR (qPCR) environmental DNA assays targeting the cytochrome c oxidase 1 (COI) and cytochrome b (cyt b) genes. Neither assay amplified DNA from the closely-related shortnose sturgeon Acipenser brevirostrum, but species-specificity should be tested further. In field and laboratory trials, results from the cyt b assay always corresponded with species presence; the COI assay occasionally yielded false positives. The cyt b assay also showed promise in estimating relative abundance; C_t value (the number of PCR cycles at which DNA detection exceeds the background level) decreased with density in both the field and lab.

为了协助监测加拿大和美国受保护物种species湖A鱼Acipenser fulvescens的分布和丰度，我们开发了两种针对细胞色素c氧化酶1（COI）和细胞色素的定量PCR（qPCR）环境DNA分析b（cyt b）基因。两种测定均未扩增出近亲短st st Acipenser brevirostrum的DNA ，但应进一步测试物种特异性。在野外和实验室试验中，cyt b测定的结果始终与物种的存在相对应。在COI分析偶尔产生误报。cyt b分析还显示出估计相对丰度的希望；Ç_吨值（在该DNA检测超过背景水平的PCR循环的数量）与在现场和实验室都密度降低。