The use of antibiotic agents in the treatment of infectious diseases has greatly contributed to the decrease in morbidity and mortality, but these great advances in treatment are being undermined by the rapidly increasing antimicrobial resistant organisms. Extended-spectrum beta-lactamases are enzymes hydrolyzing the beta lactam antibiotics, including third generation cephalosporins and monobactams but not cephamycins and carbapenems. They pose a serious global health threat and have become a challenge for health care providers. The aim of this research was to assess the prevalence of extended-spectrum beta-lactamase producing Escherichia coli in University of Nigeria Teaching Hospital Ituku-Ozalla Enugu and to detect the risk factors for acquisition of the resistant organism. To proffer advice on antibiotic stewardship in clinical practice and public health interventions, to curb the spread of the resistant organisms in the hospital. Out of the 200 E. coli isolates, 70 (35.00%) were confirmed positive for extended-spectrum beta-lactamase production. Fifty-three (75.7%) were from hospital acquired infections. All the isolates were resistant to ampicillin, tetracycline and chloramphenicol while 68 (97.14%) of the 70 isolates were susceptible to imipenem. BlaTEM, blaSHV and blaTEM were detected in 66 (94%) of the 70 isolates. The ESBL bla genes detected were blaCTX-M (n = 26; 37.14%), blaTEM (n = 7; 10.00%), blaSHV (n = 2; 2.86%), blaCTX-M/TEM (n = 7; 10.0%), blaCTX-M/SHV (n = 14; 20.0%) and blaCTX-M/TEM/SHV (n = 10; 14.29%). The three bla genes were not detected in 4 (5.71%) of the isolates. Recent surgery, previous antibiotic and intensive care unit admission were the associated risk factors to infections caused by extended-spectrum beta-lactamase producing E. coli. There is a high rate of infections caused by extended-spectrum beta-lactamase producing E. coli. Recent surgery, previous antibiotic and intensive care unit admission were associated risk factors.

中文翻译：

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在尼日利亚埃努古的一家三级医院中，产生广谱β-内酰胺酶的大肠杆菌的分子检测和抗生素抗性模式

在传染病的治疗中使用抗生素已极大地降低了发病率和死亡率，但是快速增长的抗药性微生物却破坏了这些巨大的进步。广谱β-内酰胺酶是水解β-内酰胺抗生素的酶，包括第三代头孢菌素和单bactams，但不包括头孢霉素和碳青霉烯。它们构成了严重的全球健康威胁，并已成为医疗保健提供者的挑战。这项研究的目的是评估尼日利亚大学教学医院Ituku-Ozalla Enugu产生大光谱β-内酰胺酶的大肠杆菌的流行率，并检测获得抗药性生物的危险因素。在临床实践和公共卫生干预措施中提供有关抗生素管理的建议，以遏制耐药菌在医院中的传播。在200株大肠杆菌中，有70株（35.00％）被证实对广谱β-内酰胺酶产生呈阳性反应。有53（75.7％）来自医院获得性感染。所有分离株均对氨苄西林，四环素和氯霉素具有抗药性，而在70个分离株中，有68个（97.14％）对亚胺培南敏感。在70株分离物中的66株（94％）中检测到BlaTEM，blaSHV和blaTEM。检测到的ESBL bla基因为blaCTX-M（n = 26; 37.14％），blaTEM（n = 7; 10.00％），blaSHV（n = 2; 2.86％），blaCTX-M / TEM（n = 7; 10.0％ ），blaCTX-M / SHV（n = 14; 20.0％）和blaCTX-M / TEM / SHV（n = 10; 14.29％）。在4个（5.71％）分离物中未检测到三个bla基因。最近的手术 先前使用抗生素和重症监护病房是导致产生大范围β-内酰胺酶的大肠杆菌感染的相关危险因素。由产生广谱β-内酰胺酶的大肠杆菌引起的感染率很高。最近的手术，先前的抗生素治疗和重症监护室入院是相关的危险因素。