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Deciphering the mitochondrial genome of Malabar snakehead, Channa diplogramma (Teleostei; Channidae)
Biologia ( IF 1.4 ) Pub Date : 2019-12-10 , DOI: 10.2478/s11756-019-00385-x
S. Chandhini , Sneha Vargheese , Siby Philip , V. J. Rejish Kumar

The whole mitogenome of the freshwater fish Channa diplogramma (Malabar snakehead), a vulnerable species of snakehead from the Western Ghats, India was sequenced for the first time. The genome is 16,571 bp in length, comprised of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a non-coding control region. All the protein-coding genes, tRNA and rRNA were located in the heavy strand except nad6 and 8 tRNAs (Glutamine, Alanine, Asparagine, Cysteine, Tyrosine, Serine, Glutamic acid and Proline) transcribed from L strand. The genome exhibited an overlapping of 7 bp in 2 different locations from 8100 to 10,342 bp. These overlaps were detected between protein-coding genes (atp8atp6, nad4lnad4). In addition, intergenic spacers of 167 bp were also present. The overall GC- and AT-skews of the H-strand mitogenome were 0.1077 and − 0.2439, respectively revealing that the H-strand had equal amounts of A and T and that the overall nucleotide composition was C skewed. All tRNA genes had cloverleaf secondary structures, while the secondary structure of tRNASer lacked a discernible dihydrouridine stem. The phylogenetic analysis of C. diplogramma shows its close similarity to C. micropeltes, native to Southeast Asia, Malay Peninsula and Indonesia.



中文翻译:

破译马拉巴尔蛇头(Channa diplogramma(Teleostei; Channidae))的线粒体基因组

淡水鱼类Channa diplogramma(马拉巴尔蛇头)(一种来自印度西高止山脉的脆弱蛇头物种)的整个有丝分裂基因组首次测序。基因组长度为16571 bp,由13个蛋白质编码基因,2个rRNA基因,22个tRNA基因和一个非编码控制区组成。除了从L链转录的nad6和8个tRNA(谷氨酰胺,丙氨酸,天冬酰胺,半胱氨酸,酪氨酸,丝氨酸,谷氨酸和脯氨酸)外,所有蛋白质编码基因,tRNA和rRNA均位于重链中。基因组在8100至10,342 bp的2个不同位置显示了7 bp的重叠。在蛋白质编码基因(atp8atp6,nad4lnad4)之间检测到了这些重叠)。另外,还存在167bp的基因间间隔子。H链有丝分裂基因组的总体GC和AT偏斜分别为0.1077和-0.2439,这表明该H链具有相等的A和T量,并且总核苷酸组成为C偏斜。所有的tRNA基因都具有苜蓿叶状二级结构,而tRNA Ser的二级结构缺少可分辨的二氢尿苷茎。C. diplogramma的系统发育分析表明,它与原产于东南亚,马来半岛和印度尼西亚的C. micropeltes非常相似

更新日期:2019-12-10
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