Seeds of Elaeagnus mollis (Elaeagnaceae) produce an edible oil and contain more vitamin E (Ve) than major oil-seed crops. Despite its economic value, there is no information on its genome sequence. Here, we used the Illumina platform to determine the seed transcriptome of E. mollis to identify the genes related to Ve biosynthesis and potential simple sequence repeat (SSR) markers. In total, 100,999 unigenes were obtained with an average length of 605 bp and N50 of 985 bp. Of these unigenes, 52,256 (51.7%) were annotated in at least one public database (NT, NR, PFAM, SwissProt, KOG, KO, and GO) in searches using blastn/x. The unigene annotation identified 15 unigenes encoding six enzymes (GGR, HPPD, HPT/VTE2, MPBQ-MT/VTE3, TC/VTE1, and γ-TMT/VTE4) putatively involved in Ve biosynthesis. In addition, 16,810 SSRs distributed in 14,057 unigenes were mined. Of these, 2820, 583, and 3423 SSRs were located in the 5′–UTR, coding sequence (CDS), and 3′–UTR regions, respectively, while the remaining 9984 SSRs had undetermined physical locations. The largest group of repeat motifs comprised mononucleotide repeats (70.76%), followed by dinucleotide (15.59%) and trinucleotide (12.10%) repeats. AG/CT (8.69%) and AAG/CTT (4.15%) were the main dinucleotide and trinucleotide repeats, respectively. Furthermore, 9597 SSR-specific primer pairs were designed. Among 100 primer pairs selected randomly to determine their usefulness, 53 proved to be efficient. To the our best of knowledge, this work is the first study of the E. mollis transcriptome and constitutes valuable genomics data for future genetic engineering studies to alter the amount of Ve. The identified potential EST-SSR markers can be used for population genetics studies and assisted-breeding of E. mollis.

沙枣（Elaeagnus mollis（Elaeagnaceae））的种子可产生食用油，并且比主要油料作物含有更多的维生素E（Ve）。尽管它具有经济价值，但没有有关其基因组序列的信息。在这里，我们使用Illumina平台确定E的种子转录组。莫利斯以鉴定与Ve生物合成相关的基因和潜在的简单序列重复（SSR）标记。总共获得了100,999个单基因，平均长度为605 bp，N50为985 bp。在这些单基因中，使用blastn / x在至少一个公共数据库（NT，NR，PFAM，SwissProt，KOG，KO和GO）中标注了52,256个（51.7％）。单基因注释识别了15种编码6种酶的单基因（GGR，HPPD，HPT / VTE2，MPBQ-MT / VTE3，TC / VTE1和γ-TMT/ VTE4），这些酶被认为与Ve生物合成有关。此外，还挖掘了分布在14,057个单基因中的16,810个SSR。其中，2820、583和3423个SSR分别位于5'–UTR，编码序列（CDS）和3'–UTR区域，而其余的9984 SSR的物理位置不确定。最大的重复基序组包括单核苷酸重复（70.76％），其次是二核苷酸（15.59％）和三核苷酸（12.10％）重复序列。AG / CT（8.69％）和AAG / CTT（4.15％）分别是主要的二核苷酸和三核苷酸重复序列。此外，设计了9597 SSR特异性引物对。在随机选择的100对引物中确定其有用性，其中53对被证明是有效的。据我们所知，这项工作是对Ë。mollis转录组并构成有价值的基因组学数据，以供将来进行基因工程研究以改变Ve的量。鉴定出的潜在EST-SSR标记可用于种群遗传学研究和E的辅助育种。油树。