Different studies to control the Rosellinia necatrix growth in vitro and avocado white root rot in vivo were performed, using two non-pathogenic R. necatrix isolates (Rn12 and Rn29) combined with fluazinam. The application of non-pathogenic isolates alone showed significant growth reduction as compared to R. necatrix pathogenic isolates in a PDA growth medium. The combination of both non-pathogenic isolates plus fluazinam at 0.01 mg l⁻¹ significantly increased the reduction of the pathogen growth in vitro. Additionally, the combination of the lowest fluazinam concentration (0.001%) plus Rn12 improved control of the disease as compared when either the same fungicide rate was used alone or this isolate was applied alone on plants, showing a similar response to the use of the highest concentration of fluazinam applied alone (0.01%). In this work, we have obtained a new, integrated biological control method against this avocado disease using non-pathogenic R. necatrix isolates combined with low doses of fluazinam.

中文翻译：

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使用非致病性玫瑰茄分离物结合低浓度的氟济南控制鳄梨白根腐烂

不同的研究来控制Rosellinia球虫体外生长并进行体内鳄梨白根腐病，使用两个非致病性R.球虫用氟啶胺组合分离物（RN12和Rn29）。与在PDA生长培养基中的necatrix致病性分离株相比，单独施用非病原性分离株显示出明显的生长减少。两种非致病性分离物加氟喹嗪的剂量均为0.01 mg l ^-1显着增加了体外病原体生长的减少。此外，与单独使用相同杀真菌剂率或将此分离株单独施用于植物相比，最低的氟济南浓度（0.001％）和Rn12的组合改善了对疾病的控制，显示了对最高使用率的相似反应单独应用氟西南的浓度（0.01％）。在这项工作中，我们获得了一种新的，综合的防治该鳄梨病的生物防治方法，该方法使用非致病性猪瘟分离株与低剂量的氟济南合用。