Phosphate (Pho) regulon plays a critical role in bacterial phosphate homeostasis. It is regulated by two-component system (TCS) that comprises a sensor histidine kinase and transcriptional response regulator (RR). PhoP from Enterococcus faecalis (EfPhoP) belongs to the OmpR subfamily of RRs. It has not yet been structurally characterized because it is difficult to crystallize it to full-length form. In this study, a truncated form of EfPhoP containing the receiver domain (EfPhoP-RD) was constructed, purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. The crystal of EfPhoP-RD diffracted to 3.5 Å resolution and belonged to the orthorhombic space group C2221, with unit-cell parameters a = 118.74, b = 189.83, c = 189.88 Å. The asymmetric unit contains approximately 12 molecules, corresponding to a Matthews coefficient (Vm) of 2.50  Å3 Da−1 with a solvent content of 50.9%.

中文翻译：

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粪肠球菌ATCC 29212的应答调节因子PhoP的受体域的过表达，纯化，结晶和初步X射线晶体学表征

磷酸盐（Pho）调节剂在细菌磷酸盐体内平衡中起关键作用。它由两组分系统（TCS）调节，该系统包括传感器组氨酸激酶和转录反应调节剂（RR）。粪肠球菌的PhoP（EfPhoP）属于RR的OmpR亚家族。由于很难将其结晶成全长形式，因此尚未对其进行结构表征。在这项研究中，构建了包含受体结构域（EfPhoP-RD）的截短形式的EfPhoP，纯化至均质并使用悬滴蒸气扩散法结晶。EfPhoP-RD晶体衍射至3.5Å分辨率，属于正交晶体空间群C2221，单位晶胞参数a = 118.74，b = 189.83，c = 189.88Å。不对称单元包含约12个分子，