Callose is cell wall constitutive component of some plant tissues and it is synthesized and deposited in response to stresses (abiotic and biotic). Considering physiological seed quality as an attribute that determines the rapid and uniform emergence of seedlings under a wide range of environmental conditions, this study verified the callose accumulation in seedling roots of soybean cultivars showing contrasting seed vigor under water deficit. Six soybean cultivars were used and the physiological seed quality was determined by germination and vigor tests. Water deficit simulation was performed by germination tests with polyethylene glycol solution at − 0.4 MPa, and the control (0.0 MPa; deionized water). 24 h after radicle protrusion, three samples of 13 root tips were prepared for callose determination. The cultivar ‘BMX Potência RR’ presented lower germination (87%) when compared to the other cultivars. Vigor results allowed the separation of cultivars into two vigor categories, high and low. The callose concentration deposited on soybean root tissues ranged from 0.0511 to 0.1244 μg per root tip. This allowed the observation of the contrast in callose accumulation between the cultivars during the water deficit, and this is responsive to the greater susceptibility of low vigor cultivars to stress in order to isolate plant tissue through the deposition of a physical barrier. Callose accumulation can be used as indicative of susceptibility to water stress in soybean cultivars.

中文翻译：

---

水分亏缺条件下大豆幼苗根中的愈伤组织积累

ose质是某些植物组织的细胞壁组成成分，它是根据胁迫（非生物和生物）合成和沉积的。考虑到生理种子质量是决定在各种环境条件下幼苗快速和均匀出苗的属性，本研究验证了大豆品种幼苗根中的call愈积累，在缺水条件下显示出相反的种子活力。使用了六个大豆品种，通过发芽和活力试验确定了生理种子质量。通过在-0.4 MPa的聚乙二醇溶液和对照（0.0 MPa;去离子水）的发芽试验中进行水分亏缺模拟。胚根伸出后24小时，准备了13个根尖的三个样品用于ose的测定。与其他品种相比，“ BMXPotênciaRR”品种发芽率较低（87％）。活力结果允许将品种分为高活力和低活力两个类别。每个根尖上沉积在大豆根部组织上的ose质浓度为0.0511至0.1244μg。这使得可以观察到缺水期间各品种之间call愈积累的对比，并且这响应于低活力品种对胁迫的更大敏感性，以便通过物理屏障的沉积来分离植物组织。愈伤组织的积累可以用作指示大豆品种对水分胁迫的敏感性。每个根尖上沉积在大豆根部组织上的ose质浓度为0.0511至0.1244μg。这使得可以观察到缺水期间各品种之间call愈积累的对比，并且这响应于低活力品种对胁迫的更大敏感性，以便通过物理屏障的沉积来分离植物组织。愈伤组织的积累可以用作指示大豆品种对水分胁迫的敏感性。每个根尖上沉积在大豆根部组织上的ose质浓度为0.0511至0.1244μg。这使得可以观察到缺水期间各品种之间call愈积累的对比，并且这响应于低活力品种对胁迫的更大敏感性，以便通过物理屏障的沉积来分离植物组织。愈伤组织的积累可以用作指示大豆品种对水分胁迫的敏感性。