Recently, the multi-screening of target materials has been made possible by the development of the surface plasmon resonance (SPR) imaging method. To adapt this method to biochemical analysis, the multi-patterning technology of protein microarrays is required. Among the different methods of fabricating protein microarrays, the microfluidic platform was selected due to its various advantages over other techniques. Microfluidic devices were designed and fabricated with polydimethylsiloxane (PDMS) by the replica molding method. These devices were designed to operate using only capillary force, without the need for additional flow control equipment. With these devices, multiple protein-patterned sensor surfaces were made, to support the two-dimensional detection of various protein-protein interactions with SPR. The fabrication technique of protein microarrays can be applied not only to SPR imaging, but also to other biochemical analyses.

中文翻译：

---

用聚二甲基硅氧烷微流控装置制备多组分蛋白质微阵列

最近，通过表面等离振子共振（SPR）成像方法的发展，对目标材料进行多重筛选成为可能。为了使该方法适合生化分析，需要蛋白质微阵列的多模式技术。在制造蛋白质微阵列的不同方法中，由于其相对于其他技术的各种优势，因此选择了微流体平台。通过复制成型方法，用聚二甲基硅氧烷（PDMS）设计和制造微流体装置。这些设备设计为仅使用毛细作用力运行，而无需其他流量控制设备。使用这些设备，可以制作多个蛋白质图案的传感器表面，以支持二维检测各种蛋白质与SPR的相互作用。