Sugarcane (Saccharum species hybrid) is an important cash crop and is the major source of sugar worldwide. Very little attempt has been made on optimization of conditions like temperature, humidity and light intensity for acclimatization of regenerated plantlets through micropropagation for rapid and high multiplication of disease-free planting material. In this regard, we have carried out an experiment with an objective to know the effect of temperature, light intensity and humidity on hardening of 16 elite sugarcane genotypes under three different hardening conditions, viz. single shade net, double shade net and polyhouse conditions. The single shade net condition was found optimum for shoot morphological and root characteristics in 13 tested sugarcane genotypes. However, the double shade net condition was found optimum, in genotypes VCF 0517, CoVC 07-06-05 and CoVC 07-34-05. The average temperature 28.3 °C, relative humidity 63.20% and light intensity (7522 lx) found to be best for 13 sugarcane genotypes for their shoot morphological and root characteristics under singe shade net within a short period of time.

中文翻译：

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温度，湿度和光照强度对微繁殖甘蔗（蔗糖杂种）基因型的影响

甘蔗（甘蔗杂种）是重要的经济作物，并且是全球糖的主要来源。对于通过微繁殖使无病种植材料快速大量繁殖而使再生小苗适应环境，诸如温度，湿度和光照强度等条件的优化方面，几乎没有进行任何尝试。在这方面，我们进行了一项实验，目的是了解温度，光强度和湿度在三种不同的硬化条件下对16种优良甘蔗基因型硬化的影响。单遮阳网，双遮阳网和合棚条件。发现单一阴影网状条件最适合13个测试的甘蔗基因型的芽形态和根部特征。但是，在VCF 0517号基因型中，发现双阴影网状条件是最佳的，CoVC 07-06-05和CoVC 07-34-05。研究发现，平均温度28.3°C，相对湿度63.20％和光照强度（7522 lx）最适合13个甘蔗基因型，因为它们在短时间内在单一阴影下的枝条形态和根部特性。