The current study was conducted to evaluate the efficacy of entomopathogenic fungi (EPF) as an alternative strategy for the sustainable control of Nilaparvata lugens. Three species of EPF, Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii, were tested against N. lugens with two suspensions of each tested EPF applied in different treatments. The observed mortality of N. lugens adults during the overall exposure period for the lowest and highest concentrations of each EPF ranged from 0–100%. At both highest and lowest concentrations of EPF, when sprayed on adult, the mortality of N. lugens was higher as compared to when sprayed on the stem pieces as food. A higher mortality rate was observed when the stem pieces were absent than when stem pieces were present. Maximum percent mortality of N. lugens was recorded due to the spray of highest concentration of M. anisopliae on only stem pieces (82.67%) and on adult N. lugens provided with stem pieces as food (93.33%) after 14 days of exposure interval. On the other hand, B. bassiana and L. lecanii were also responsible to cause more than 50% mortality of N. lugens in both treatments after 14 days of exposure interval. Results also indicated that maximum and minimum percent mycosis and sporulation from dead cadavers of N. lugens were recorded in both conidial suspensions of L. lecanii and M. anisopliae, respectively, in all treatments. The high efficacy levels recorded in the current study indicates that M. anisopliae can be effective biological control agents against N. lugens.

中文翻译：

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病原真菌对褐飞虱Nilaparvata Lugens（Stål）（Homoptera：Delphacidae）在控制条件下的功效

进行当前的研究以评估昆虫病原性真菌（EPF）作为可持续控制褐飞虱的替代策略的功效。三种EPF的白僵菌，金龟子绿僵菌和蜡蚧枝菌三级，进行了对所测试的褐飞虱与每个测试EPF的两种悬浮液在不同的治疗应用。在整个暴露期间，每种EPF的最低和最高浓度下，成人猪笼草的死亡率均在0-100％之间。在最高和最低浓度的EPF上，当对成年男子喷洒时，黄褐猪笼草的死亡率与作为食物喷在茎上时相比更高。当没有茎杆时，观察到的死亡率高于存在茎杆时的死亡率。由于暴露间隔14天后仅在茎杆上（82.67％）和以茎杆为食物的成年N. lugens喷洒了最高浓度的M. anisopliae，因此记录了N. lugens的最大死亡率百分比。。另一方面，在暴露间隔14天后，两种处理中，球孢杆菌和莱卡氏乳杆菌也可导致超过50％的猪笼草死亡。结果还表明死于尸体的真菌病和孢子形成的最大和最小百分比在所有处理中，分别在lecanii L. and anisopliae的分生孢子悬浮液中记录了N. lugen。目前研究中记录的高功效水平表明，异枝线虫可以作为有效的防治褐飞虱的生物防治剂。