ABSTRACT The leccinoid fungi are boletes and related sequestrate mushrooms (Boletaceae, Basidiomycota) that have traditionally been placed in Leccinum, Boletus, Leccinellum, and a handful of other less familiar genera. These mushrooms generally feature scabers or scaber-like dots on the surface of the stipe, and they are often fairly tall and slender when compared with other boletes. They are ectomycorrhizal fungi and appear to be fairly strictly associated with specific trees or groups of related trees. In the present study, we investigate the phylogenetic relationships among the leccinoid fungi and other members of the family Boletaceae using portions of three loci from nuc 28S rDNA (28S), translation elongation factor 1-α (TEF1), and the RNA polymerase II second-largest subunit (RPB2). Two DNA data sets (combined 28S-TEF1 and 28S-TEF1-RPB2), comprising sequences from nearly 270 voucher specimens, were evaluated using two different phylogenetic analyses (maximum likelihood and Bayesian inference). Five major clades were obtained, and leccinoid fungi appeared in four of them. Taxonomic proposals supported by our results, representing a broadly circumscribed Leccinum that includes several sequestrate genera, along with Leccinellum, are made.

中文翻译：

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基于分子和形态学数据，修正类球虫真菌，重点是北美分类群

摘要 leccinoid 真菌是牛肝菌和相关的螯合蘑菇（牛肝菌科，担子菌门），传统上被放置在 Leccinum、牛肝菌属、Leccinellum 和少数其他不太熟悉的属中。这些蘑菇的菌柄表面通常有疤痕或疤痕状的点，与其他牛肝菌相比，它们通常相当高和细长。它们是外生菌根真菌，似乎与特定的树或相关树群有相当严格的关联。在本研究中，我们使用来自 nuc 28S rDNA (28S)、翻译延伸因子 1-α (TEF1) 和第二个 RNA 聚合酶 II 的三个基因座的部分研究了 leccinoid 真菌和牛肝菌科其他成员之间的系统发育关系-最大亚基（RPB2）。两个 DNA 数据集（组合 28S-TEF1 和 28S-TEF1-RPB2），包含来自近 270 个凭证样本的序列，使用两种不同的系统发育分析（最大似然和贝叶斯推理）进行评估。获得了五个主要进化枝，其中四个出现了类卵磷脂真菌。我们的结果支持的分类学建议代表了一个广泛限制的 Leccinum，其中包括几个螯合属，以及 Leccinellum。