γ-Aminobutyric acid (GABA) is recognised as a potential metabolic bioactive food ingredient with increasing evidence of its effects on the gut-brain axis and systemic metabolic health. Different lactic acid bacteria are capable of producing GABA, particularly strains of Lactobacillus brevis. In this study, we characterized a Lb. brevis isolated from traditional alpine cheese (Lb. brevis DSM 32386) for its ability to accumulate high levels of GABA in the culture medium and for other important probiotic phenotypic traits. In vitro analysis were used to study the Lb. brevis DSM 32386 probiotic traits and the gene expression involved in GABA production Lactobacillus brevis DSM 32386 converted monosodium glutamate to GABA more efficiently than the type strain Lb. brevis DSM 20054, resulting in more than 200% of GABA produced. This ability seemed to be related to the higher transcriptional activation of the gene encoding for the glutamate (gad) decarboxylase antiporter (gadC) and regulator (gadR). Lactobacillus brevis DSM 32386 performed well in vitro under the stress conditions mimicking the gastro-intestinal tract, being resistant to acid pH (pH 2.5) and growing in simulated pancreatic fluid and 0.3% ox-bile. These preliminary studies indicate that Lb. brevis DSM 32386 holds promise as a starter for GABA-rich dairy fermented foods and possibly a promising next-generation probiotic microorganism in the context of the gut (microbiota):brain axis.

中文翻译：

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在体外高GABA生产菌株的益生菌表征Lactobacilluas短乳杆菌DSM 32386从传统的“野生”阿尔卑斯乳酪分离

γ-氨基丁酸（GABA）被认为是潜在的代谢生物活性食品成分，其对肠脑轴和全身代谢健康的影响的证据越来越多。不同的乳酸菌能够产生GABA，特别是短乳杆菌菌株。在这项研究中，我们表征了Lb。从传统的高山奶酪（Lb. brevis DSM 32386）中分离出来的brevis，因为它能够在培养基中积累高水平的GABA以及其他重要的益生菌表型性状。体外分析用于研究Lb。brevis DSM 32386益生菌性状和参与GABA产生的基因表达brevis DSM 32386比Lb型菌株更有效地将谷氨酸一钠转化为GABA。brevis DSM 20054，产生超过200％的GABA。这种能力似乎与编码谷氨酸（gad）脱羧酶反转运蛋白（gadC）和调节剂（gadR）的基因的更高转录激活有关。短乳杆菌DSM 32386在模拟胃肠道的应激条件下体外表现良好，对酸性pH（pH 2.5）有抵抗力，并在模拟的胰液和0.3％的牛胆汁中生长。这些初步研究表明，Lb。brevis DSM 32386有望成为富含GABA的乳制品发酵食品的发源地，并且有可能在肠道（微生物群）：脑轴的背景下成为下一代有前景的益生菌微生物。短乳杆菌DSM 32386在模拟胃肠道的应激条件下体外表现良好，对酸性pH（pH 2.5）有抵抗力，并在模拟的胰液和0.3％的牛胆汁中生长。这些初步研究表明，Lb。brevis DSM 32386有望成为富含GABA的乳制品发酵食品的发源地，并且有可能在肠道（微生物群）：脑轴的背景下成为下一代有前景的益生菌微生物。短乳杆菌DSM 32386在模拟胃肠道的应激条件下体外表现良好，对酸性pH（pH 2.5）有抵抗力，并在模拟的胰液和0.3％的牛胆汁中生长。这些初步研究表明，Lb。brevis DSM 32386有望成为富含GABA的乳制品发酵食品的发源地，并且有可能在肠道（微生物群）：脑轴的背景下成为下一代有前景的益生菌微生物。