Diospyros lasiocalyx (Mart.) B. Wall. is a dioecious tree species found in the threatened and fragmented Brazilian Cerrado. Its fleshy fruits, which are consumed extensively by wildlife, make the species ideal for use in restoration. This study includes a ten-year phenological observation, germination tests, and analysis of molecular markers to understand the reproductive strategy of D. lasiocalyx based on samples from three populations in two Cerrado ecosystems in São Paulo State, Brazil. The within population reproductive phenological pattern presented low synchrony, indicating a lack of sexual reproduction. The vegetative phenological pattern was well pronounced, with the peak of senescence occurring at the end of the dry season, just before leaf flushing. A small number of unique genets were found, indicating asexual reproduction and low genotypic diversity in the studied populations. The absence of inbreeding and the presence of ramets (stems) suggest that reproduction mainly occurs by apomixis and vegetative propagation, with some mating among unrelated stems and a low germination rate. Our study indicates that D. lasiocalyx can colonize harsh environments such as the Cerrado using alternative asexual reproductive strategies, while simultaneously attracting many seed dispersers.

中文翻译：

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物候，种子萌发和遗传学解释了柿（B. Wall。Diospyros lasiocalyx）的繁殖策略。

Diospyros lasiocalyx（Mart。）B.沃尔。是在濒临灭绝的巴西塞拉多地区发现的一种雌雄异株树种。其多肉的水果被野生动植物广泛消费，使该物种成为恢复的理想之选。这项研究包括十年的物候观察，发芽测试和分子标记物分析，以了解D. lasiocalyx的繁殖策略。基于巴西圣保罗州两个Cerrado生态系统中三个种群的样本。人口内部生殖物候模式表现出较低的同步性，表明缺乏性生殖。营养物候模式非常明显，衰老的高峰出现在干旱季节结束时，即在潮红之前。发现了少数独特的种系，表明在研究人群中无性繁殖和低基因型多样性。没有近亲繁殖和分株（茎）的存在表明繁殖主要通过无融合生殖和无性繁殖而发生，在不相关的茎中有一些交配并且发芽率低。我们的研究表明，D。lasiocalyx 可以使用其他无性繁殖策略在诸如塞拉多（Cerrado）等恶劣环境中定殖，同时吸引许多种子传播者。