The diversity of lactic acid bacteria (LAB) in silages produced in warm climate countries is not well known. This study aimed to identify and characterise the metabolic and genotypic aspects of autochthonous LAB isolated from corn silage produced in the state of Minas Gerais, Brazil. Eighty-eight LAB were isolated. To evaluate their performance at the strain level, all isolates were distinguished among strains using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and repetitive extragenic palindromic PCR (REP-PCR) techniques. The organic acid and ethanol production were determined by high-performance liquid chromatography (HPLC). The fingerprints obtained by RAPD-PCR with a M13 primer were more discriminatory than those obtained with the REP-PCR technique using a (GACA)4 primer. Moreover, 28 representative isolates were identified as Lactobacillus acidophilus, L. buchneri, L. casei, L. diolivorans, L. hilgardii, L. paracasei, L. parafarraginis, L. plantarum, L. rhamnosus, L. zeae and Pediococcus acidilactici. Different fingerprinting profiles between isolates within the same species were observed. However, some strains isolated from different silages showed the same band profile, thus suggesting the presence of clusters with high similar fingerprints in silages from various regions. A variation in LAB diversity was observed in the silages of the evaluated regions, with L. rhamnosus and L. buchneri showing the highest distribution. Differences in organic acid production were observed among the strains belonging to the same species. This research contributes to a better understanding of the LAB community present in corn silage produced in warm climates. These strains will be studied as potential silage starters.

中文翻译：

---

米纳斯吉拉斯州（巴西）生产的玉米青贮饲料中乳酸菌的多样性

温暖气候国家生产的青贮饲料中乳酸菌（LAB）的多样性并不为人所知。这项研究旨在鉴定和表征从巴西米纳斯吉拉斯州生产的玉米青贮饲料中分离得到的本地LAB的代谢和基因型特征。分离出88个LAB。为了评估其在菌株水平上的性能，使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）和重复性外源回文PCR（REP-PCR）技术区分了所有菌株。通过高效液相色谱法（HPLC）测定有机酸和乙醇的产量。通过RAPD-PCR使用M13引物获得的指纹比使用（GACA）4引物的REP-PCR技术获得的指纹更具区分性。此外，鉴定出28个代表性分离株为嗜酸乳杆菌，布氏乳杆菌，干酪乳杆菌，多烯乳杆菌，hilgardii，副干酪乳杆菌，副干酪乳杆菌，植物乳杆菌，鼠李糖乳杆菌，玉米乳杆菌和嗜酸乳杆菌。观察到同一物种内分离株之间的指纹图谱不同。但是，从不同青贮饲料中分离出的某些菌株显示出相同的条带图谱，因此表明在各个地区的青贮饲料中都存在具有高度相似指纹的簇。在评价区域的青贮饲料中观察到了LAB多样性的变化，鼠李糖乳杆菌和布氏乳杆菌显示出最高的分布。在属于同一物种的菌株之间观察到有机酸产生的差异。这项研究有助于更好地了解温暖气候下生产的玉米青贮饲料中的乳酸菌群落。这些菌株将作为潜在的青贮饲料开始研究。