The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host’s cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA₁₂₀ - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms’ surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA₁₂₀ which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC₅₀ 1.81 μg mL⁻¹ and to P. roqueforti with 1.10 μg mL⁻¹. This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.

中文翻译：

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基于ELLSA的微生物表面糖基化分析表明，富含β-葡聚糖的酵母表面可通过重组香蕉凝集素选择性识别

微生物的表面覆盖有多糖结构，该多糖结构立即与宿主细胞和抗体上的受体结构接触。微生物与其宿主之间的相互作用取决于表面糖基化，在这项研究中，我们测试了植物凝集素与不同微生物的相互作用。酶联凝集素吸附剂测定-ELLSA用于测试重组尖头Musa凝集素-BL与27种选定微生物的结合，另外7种凝集素用于比较：大豆凝集素-SBA，Lens culinaris凝集素-LCA，小麦胚芽凝集素- WGA，RCA ₁₂₀ -蓖麻凝集素，刀豆A -从刀豆，接骨木花黑凝集素-SNA I和黑莓（Maackia amurensis）凝集素-MAA。目的是通过与所选植物凝集素的相互作用来定义微生物的表面糖基化，并与BL进行比较。在测试的凝集素中，对于RCA ₁₂₀观察到最强的选择性结合，后者优先结合干酪乳杆菌DG。重组香蕉凝集素显示出与所有测试真菌物种的特异性结合。用荧光显微镜和流式细胞术进一步测试了BL与白色念珠菌的结合，并得出结论，该凝集素可以区分富含β-葡聚糖的表面。BL与布拉氏链霉菌的结合可以与β-葡聚糖从酵母能够抑制带IC ₅₀ 1.81微克毫升^-1和P.娄地与1.10微克毫升^-1。BL的这种独特特异性可用于筛选目的，并有可能用于检测溶液中的β-葡聚糖。