Here, we explore the responses of photosynthesis and related cellular processes in the thermotolerant microalga Micractinium sp. acclimated to limiting and saturating irradiances combined with elevated temperatures, using a novel computer‐controlled multi‐sensor system. This system allows for the monitoring of online values of oxygen exchange during photosynthesis and respiration with high accuracy. Micractinium sp. cells showed maximum growth and net oxygen production rates under the optimal temperature of 25°C regardless of the light acclimation conditions. Our results show that the upper thermal threshold for Micractinium sp. photosynthesis and growth ranges between 35°C and 40°C. This microalga exhibited stable photosynthetic efficiency and effective non‐photochemical quenching (NPQ) under saturating light, and was more susceptible to temperature change when acclimated to limiting light levels. These results demonstrate that the acclimation of thermotolerant microalgae to saturating light helps to enhance the thermal tolerance of PSII. This feature results from enhanced heat stability of PSII photochemistry and oxygen evolution.

中文翻译：

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耐热藻适应光和温度的相互作用1。

在这里，我们探索耐热微藻Micractinium sp中光合作用和相关细胞过程的响应。使用新型计算机控制的多传感器系统，可以适应辐照度的限制和饱和以及升高的温度。该系统允许以高精度监测光合作用和呼吸过程中氧气交换的在线值。Micractinium sp。不论光照条件如何，在25°C的最佳温度下，细胞均显示出最大的生长和净氧气产生速率。我们的结果表明，菌根的最高热阈值sp。光合作用和生长范围在35°C至40°C之间。这种微藻在饱和光下表现出稳定的光合作用效率和有效的非光化学猝灭（NPQ），并且在适应极限光照水平时更容易受到温度变化的影响。这些结果表明，使耐热微藻适应光的饱和有助于增强PSII的耐热性。此功能归因于PSII光化学的热稳定性增强和氧气释放。