This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using this system to fabricate biomaterials. Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation. Based on the results of a live-dead assay, more than 80% of cell viability has been confirmed which was reasonably similar to that in the control group. Furthermore, measurement of cell metabolic activity after dispensing confirmed that the dispensed cell proliferated at a rate comparable to that of the control group. These results demonstrate that the pneumatic dispensing system is a promising tool for fabrication of biomaterials.

中文翻译：

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使用小鼠3T3-J2成纤维细胞的生物相容性气动分配系统的可行性研究

本文介绍了使用气动分配系统分配活细胞的结果，以验证使用该系统制造生物材料的可行性。为活细胞（即小鼠3T3-J2成纤维细胞）分配不同的分配压力，以评估分配过程对细胞活力和增殖的影响。根据活死分析的结果，已确认超过80％的细胞生存力与对照组相当。此外，在分配之后对细胞代谢活性的测量证实了所分配的细胞以与对照组相当的速率增殖。这些结果表明，气动分配系统是用于制造生物材料的有前途的工具。