Chemical modification of magnetic nanoparticles (MNPs) with functional polymers has recently gained a great deal of attention because of the potential application of MNPs toin vivo andin vitro biotechnology. The potential use of MNPs as capturing agents and sensitive biosensors has been intensively investigated because MNPs exhibit good separation-capability and binding-specificity for biomolecules after suitable surface functionalization processes. In this work, we demonstrate an efficient method for the surface modification of MNPs, by combining surface-initiated polymerization and the subsequent conjugation of the biologically active molecules. The polymeric shells of non-biofouling poly(poly(ethylene glycol) methacrylate) (pPEGMA) were introduced onto the surface of MNPs by surface-initiated, atom transfer radical polymerization (SI-ATRP). With biotin as a model of biologically active compounds, the polymeric shells underwent successful post-functionalization via activation of the polymeric shells and bioconjugation of biotin. The resulting MNP hybrids showed a biospecific binding property for streptavidin and could be separated by magnet capture.

中文翻译：

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聚（聚（甲基丙烯酸乙二醇酯）包被的氧化铁磁性纳米粒子的生物共轭）用于靶蛋白的磁性捕获

功能性高分子对磁性纳米粒子（MNPs）的化学修饰近来备受关注，因为MNPs可能在体内和体外应用生物技术。由于在适当的表面功能化过程之后，MNP对生物分子表现出良好的分离能力和结合特异性，因此对MNP作为捕获剂和敏感生物传感器的潜在用途进行了深入研究。在这项工作中，我们通过结合表面引发的聚合反应和随后的生物活性分子结合，证明了MNPs表面修饰的有效方法。通过表面引发的原子转移自由基聚合（SI-ATRP），将非生物污损的聚甲基丙烯酸聚乙二醇酯（pPEGMA）的聚合物壳引入MNP的表面。使用生物素作为生物活性化合物的模型，聚合物壳通过激活聚合物壳和生物素的生物结合而成功地进行了后功能化。