The tomatoMi-1gene mediates plant resistance to whiteflyBemisia tabaci, nematodes, and aphids. Other genes are also required for this resistance, and a model of interaction between the proteins encoded by these genes was proposed. Microarray analyses were used previously to identify genes involved in plant resistance to pests or pathogens, but scarcely in resistance to insects. In the present work, the GeneChip™ Tomato Genome Array (Affymetrix®) was used to compare the transcriptional profiles of Motelle (bearingMi-1) and Moneymaker (lackingMi-1) cultivars, both before and afterB. tabaciinfestation. Ten transcripts were expressed at least twofold in uninfested Motelle than in Moneymaker, while other eight were expressed half or less. After whitefly infestation, differences between cultivars increased to 14 transcripts expressed more in Motelle than in Moneymaker and 14 transcripts less expressed. Half of these transcripts showed no differential expression before infestation. These results show the baseline differences in the tomato transcriptomic profile associated with the presence or absence of theMi-1gene and provide us with valuable information on candidate genes to intervene in either compatible or incompatible tomato–whitefly interactions.

中文翻译：

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番茄叶片转录谱的基础差异与抗性基因 Mi-1 的存在/缺失相关，以及在粉虱Bemisia tabaci 侵染后这些差异的变化

番茄米一1基因介导植物对粉虱的抗性烟粉虱、线虫和蚜虫。这种抗性还需要其他基因，并且提出了由这些基因编码的蛋白质之间的相互作用模型。微阵列分析以前用于鉴定与植物对害虫或病原体的抗性有关的基因，但很少用于对昆虫的抗性。在目前的工作中，GeneChip™ 番茄基因组阵列（Affymetrix®）用于比较Motelle（轴承米一1) 和 Moneymaker (缺乏米一1) 栽培品种，之前和之后烟粉虱侵扰。十个转录本在未受感染的 Motelle 中的表达量至少是 Moneymaker 的两倍，而其他八个转录本的表达量为一半或更少。在粉虱侵染后，品种之间的差异增加到 14 个转录本在 Motelle 中的表达比在 Moneymaker 中表达的多，而 14 个转录本的表达较少。这些转录本中有一半在感染前没有表现出差异表达。这些结果显示了番茄转录组谱的基线差异与是否存在米一1基因并为我们提供有关候选基因的有价值信息，以干预相容或不相容的番茄-粉虱相互作用。