Scientific information regarding the microbial content and functional aspects of fermented beverages traditionally produced in certain parts of Europe are scarce. However, such products are believed to have some health benefits and might contain functional bacterial strains, such as probiotics. The aim of the study was to identify such lactic acid bacteria strains isolated from water kefir and, for the first time, from braga, a Romanian fermented beverage made of cereals. Lactic acid bacteria (LAB) were identified to species level based on (GTG)5-PCR fingerprinting and 16S rRNA gene sequencing. Selected strains were screened for their antibacterial activity and probiotic potential. Eight isolates belonging to seven Lactobacillus species were recovered from the two drinks. The identification of LAB involved in the fermentation of braga (Lactobacillus plantarum, Lactobacillus fermentum, and Lactobacillus delbrueckii) is firstly reported here. Five of the Lactobacillus isolates showed antibacterial activity against pathogenic bacteria, including Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Salmonella enterica. Moreover, most of them showed a good resistance to pH 2.5 and some survived at high concentrations of bile salts (up to 2%). Two L. plantarum isolates were able to inhibit all the indicator strains, and showed the best viability (about 70%) after a sequential treatment simulating the passage through the gastrointestinal tract. Based on the results, the most promising candidates for designing new probiotic products are: L. plantarum BR9 from braga and L. plantarum CR1 from water kefir.

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从两种不同发酵饮料中分离出的乳酸菌的鉴定和益生菌性质

关于欧洲某些地区传统上生产的发酵饮料的微生物含量和功能方面的科学信息很少。但是，这些产品被认为具有一定的健康益处，并且可能包含功能性细菌菌株，例如益生菌。该研究的目的是鉴定从开菲尔酒中分离出的乳酸菌菌株，并首次从罗马尼亚谷物发酵饮料braga中分离出乳酸菌。基于（GTG）5-PCR指纹图谱和16S rRNA基因测序，将乳酸菌（LAB）鉴定到物种水平。筛选所选菌株的抗菌活性和益生菌潜力。从两种饮料中回收了八个属于七个乳杆菌属的菌株。首次报道了布拉格酵母（植物乳杆菌，发酵乳杆菌和德氏乳杆菌）发酵中的乳酸菌的鉴定。乳酸杆菌中有五种对病原菌具有抗菌活性，包括单核细胞增生性李斯特菌，大肠杆菌，金黄色葡萄球菌和肠炎沙门氏菌。此外，它们中的大多数显示出对pH 2.5的良好抵抗力，并且一些在高浓度的胆汁盐（高达2％）下还可以存活。两个植物乳杆菌分离物能够抑制所有指示菌株，并且在模拟通过胃肠道的顺序治疗后显示出最佳的生存力（约70％）。根据结果​​，设计新益生菌产品的最有前途的候选者是：布拉加的L. plantarum BR9和水开菲尔的L. plantarum CR1。