Abstract A method for the simultaneous determination of glyphosate and aminomethylphosphonic acid (AMPA) in urine was developed using a “dilute-and-shoot” sample preparation. A 1:1 dilution of urine sample in 15 mmol L −1 heptafluorobutyric acid (HFBA) was prepared prior to being injected into the LC–MS/MS system. The chromatographic separation was achieved by a Gemini C6-Phenyl analytical column, with the gradient elution of 15 mmol L −1 HFBA in water and acetonitrile. The limits of detection and quantitation of both compounds were 2.5 ng mL −1 and 5 ng mL −1 , respectively. The sample preparation time was achieved in 2 min using only 0.1 mL of urine sample. The validated method was successfully applied to glyphosate and AMPA determination in farmer urine samples. Graphic Abstract

中文翻译：

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一种用于尿草甘膦和 AMPA 的稀释和喷射 LC-MS/MS 方法

摘要 开发了一种同时测定尿液中草甘膦和氨甲基膦酸 (AMPA) 的方法，该方法采用“稀释-喷射”样品制备方法。在注入 LC-MS/MS 系统之前，制备了用 15 mmol L -1 七氟丁酸 (HFBA) 1:1 稀释的尿样。色谱分离通过Gemini C6-Phenyl分析柱实现，梯度洗脱为15 mmol L -1 HFBA的水和乙腈溶液。两种化合物的检测限和定量限分别为2.5 ng mL -1 和5 ng mL -1 。样品制备时间仅需 0.1 mL 尿样即可在 2 分钟内完成。经验证的方法已成功应用于农民尿样中草甘膦和AMPA的测定。图形摘要