This study aims to utilize tuna condensate as a renewable resource for production of antifungal compounds by Streptomyces philanthi RL-1-178 against aflatoxingenic B₁ (AFB₁) Aspergillus flavus. Among ten isolates of A. flavus tested, the strain PSRDC-4 was the most toxigenic strain (1432 ppb of AFB₁) and aggressive to S. philanthi (85.9% inhibition). The effective dose (100% inhibition) of the culture filtrate RL-1-178 was at 10.0% (v/v) with 1 h exposure time. The antifungal compounds, identified by GC–MS analysis, were consisted of 105 components with 2,4-imidazolidinedione (31.2%) followed by acetic acid (25.27%) was the most abundant. The optimum condition for growth and production of antifungal compounds from S. philanthi RL-1-178 was as following; tuna condensate of 15,000 mg L⁻¹ COD, the initial pH at 7.0, incubation temperature at 30 °C and supplemented with 8 g L⁻¹ of molasses. Therefore, tuna condensate exhibited a high potential to be utilized as an alternative medium for antifungal production by S. philanthi RL-1-178.

中文翻译：

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金枪鱼冷凝物废糖蜜作为抗真菌化合物的可再生底物，由链霉菌RL-1-178对抗黄曲霉毒素B 1（AFB 1）黄曲霉

这项研究旨在利用金枪鱼冷凝物作为可再生资源，通过链霉菌RL-1-178对黄曲霉毒素B ₁（AFB ₁）黄曲霉产生抗真菌化合物。中的10株黄曲霉测试，应变PSRDC-4是最产毒菌株（1432 ppb的AFB的₁）和攻击性S. philanthi（抑制率为85.9％）。培养液RL-1-178的有效剂量（100％抑制）为10.0％（v / v），暴露时间为1小时。通过GC-MS分析鉴定出的抗真菌化合物由105种成分组成，其中2,4-咪唑烷二酮（占31.2％），其次是乙酸（占25.27％）。从费氏链球菌RL-1-178生长和生产抗真菌化合物的最佳条件如下：15,000 mg L ^-1 COD的金枪鱼冷凝物，初始pH值为7.0，孵育温度为30°C，并补充了8 g L ^-1的糖蜜。因此，金枪鱼冷凝表现出以用作通过用于抗真菌生产的替代介质的高电位S. philanthi RL-1-178。