Aerobiology studies biological particles present in the air of both indoor and outdoor environments. In the past few years, the application of molecular techniques has considerably improved the knowledge of organismal diversity in air samples and highlighted its ecological and clinical importance. DNA metabarcoding in particular has been recently applied worldwide to describe the biological composition of airborne bacteria, fungi and plants. Here, we provide a comprehensive overview of those aerobiology studies which, in the past few years, have applied DNA metabarcoding techniques. We focus specifically on researches including plants and fungi and review the multiplicity of experimental plans, sampling procedures, laboratory processing methods, the choice of the DNA barcode(s), the selection of sequencing platforms and bioinformatics pipelines for the comparability of data obtained from multiple origins.

中文翻译：

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植物和真菌 DNA 宏条形码在空气生物学中的相关性

空气生物学研究存在于室内和室外环境空气中的生物颗粒。在过去几年中，分子技术的应用大大提高了对空气样本中生物多样性的认识，并突出了其生态和临床重要性。特别是 DNA 元条形码最近已在世界范围内应用于描述空气中细菌、真菌和植物的生物组成。在这里，我们全面概述了在过去几年中应用了 DNA 元条形码技术的那些空气生物学研究。我们特别关注包括植物和真菌在内的研究，并审查实验计划的多样性、采样程序、实验室处理方法、DNA 条形码的选择、