Acute coronary syndrome (ACS) is a clinical condition caused by a disturbance in myocardial blood flow. ACS can be basically divided into two forms: ST elevation myocardial infarction (STEMI) due to complete occlusion of the coronary artery and non-ST elevation myocardial infarction (NSTEMI) due to partial occlusion of the coronary artery. In this study, we aimed to monitor the metabolite profile of STEMI and NSTEMI patients and compare the results via untargeted metabolomics approach. Serum samples were collected from STEMI and NSTEMI patients, and each group consists of 20 participants. Extraction was achieved by acetonitrile, and chromatographic separation was performed by LC/Q-TOF/MS/MS accompanied with dual AJS ESI positive ion mode. METLIN, MATLAB 2017a-PLS Toolbox7.2, and Human Metabolome Database were utilized for bioinformatics evaluation of obtained findings. In our results, 203 m/z ratio was detected and 163 m/z ratio passed the significance criteria (fold analysis > 1.5 and p < 0.05). Twenty-five metabolites including BCAAs, LysoPC species, lactic acid, succinate, malonic acid, maleic acid, butyric acid, carnitine, and betaine were identified. In conclusion, new biomarker candidates were identified to differentiate the diagnosis of STEMI and NSTEMI. Identified metabolites are indicative of alterations in oxidative stress, hypoxia, TCA cycle, and amino acid metabolism.

中文翻译：

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LC/Q-TOF/MS/MS研究ST段抬高型心肌梗死与非ST段抬高型心肌梗死的代谢差异

急性冠脉综合征 (ACS) 是一种由心肌血流紊乱引起的临床病症。ACS基本上可以分为两种形式：冠状动脉完全闭塞引起的ST段抬高型心肌梗死（STEMI）和冠状动脉部分闭塞引起的非ST段抬高型心肌梗死（NSTEMI）。在这项研究中，我们旨在监测 STEMI 和 NSTEMI 患者的代谢物谱，并通过非靶向代谢组学方法比较结果。从 STEMI 和 NSTEMI 患者收集血清样本，每组由 20 名参与者组成。乙腈提取，LC/Q-TOF/MS/MS色谱分离，双AJS ESI正离子模式。METLIN, MATLAB 2017a-PLS Toolbox7.2, 和人类代谢组数据库用于对获得的结果进行生物信息学评估。在我们的结果中，检测到 203 m/z 比率并且 163 m/z 比率通过了显着性标准（折叠分析 > 1.5 和 p < 0.05）。鉴定了 25 种代谢物，包括支链氨基酸、LysoPC 种类、乳酸、琥珀酸、丙二酸、马来酸、丁酸、肉碱和甜菜碱。总之，确定了新的候选生物标志物以区分 STEMI 和 NSTEMI 的诊断。鉴定的代谢物表明氧化应激、缺氧、TCA 循环和氨基酸代谢的改变。鉴定出琥珀酸、丙二酸、马来酸、丁酸、肉碱和甜菜碱。总之，确定了新的候选生物标志物以区分 STEMI 和 NSTEMI 的诊断。鉴定的代谢物表明氧化应激、缺氧、TCA 循环和氨基酸代谢的改变。鉴定出琥珀酸、丙二酸、马来酸、丁酸、肉碱和甜菜碱。总之，确定了新的候选生物标志物以区分 STEMI 和 NSTEMI 的诊断。鉴定的代谢物表明氧化应激、缺氧、TCA 循环和氨基酸代谢的改变。