Bacillus bacteria have major utility in large-scale production of industrial enzymes, among which proteases have particular importance. B. subtilis B22, an aerobic and chemotrophic strain, was isolated from kimchi and identified by 16S rRNA gene sequencing. Extracellular protease production was determined in basic medium, with 1% (w/v) casein as substrate, by submerged fermentation at 37 °C under blue, green, red and white light-emitting diodes (LEDs), white fluorescent light and darkness. Fermentation under blue LEDs maximized protease production (110.79 ± 1.8 U/mL at 24 h). Various agricultural waste products enhanced production and groundnut oil cake yielded the most protease (334 ± 1.8 U/mL at 72 h). Activity and stability of the purified protease were optimum at pH 7–10 and 20–60 °C. Activity increased in the presence of Ca²⁺, Mg²⁺ and Mn²⁺, while Fe²⁺, Zn²⁺, Co²⁺ and Cu²⁺ moderated activity, and Ni²⁺ and Hg²⁺ inhibited activity. Activity was high (98%) in the presence of ethylenediaminetetraacetic acid (EDTA) but inhibited by phenylmethanesulfonyl fluoride (PMSF). The protease was unaffected by nonionic surfactants, tolerated an anionic surfactant and oxidizing agents, and was compatible with multiple organic solvents. These properties suggest utility of protease produced by B. subtilis B22 under blue LEDs for industrial applications.

芽孢杆菌细菌在大规模生产工业酶中具有主要用途，其中蛋白酶特别重要。枯草芽孢杆菌从泡菜中分离出一种需氧和营养型菌株B22，并通过16S rRNA基因测序进行鉴定。通过在37°C下在蓝色，绿色，红色和白色发光二极管（LED），白色荧光灯和暗处在37°C下进行浸没发酵，在碱性培养基中以1％（w / v）酪蛋白为底物测定细胞外蛋白酶的产生。在蓝色LED下发酵可最大程度地提高蛋白酶的产量（24 h时为110.79±1.8 U / mL）。各种农业废品提高了产量，花生油饼产生的蛋白酶最多（72 h时为334±1.8 U / mL）。纯化的蛋白酶的活性和稳定性在pH 7–10和20–60°C时最佳。在Ca 2 ⁺，Mg ²⁺和Mn ²⁺的存在下，活性增加，而Fe 2 ⁺，Zn ²⁺，Co ²⁺和Cu ^2+的活性适中，而Ni ²⁺和Hg ^2+的活性受到抑制。在乙二胺四乙酸（EDTA）存在下，活性很高（98％），但被苯基甲磺酰氟（PMSF）抑制。该蛋白酶不受非离子表面活性剂的影响，可耐受阴离子表面活性剂和氧化剂，并且与多种有机溶剂相容。这些性质表明由枯草芽孢杆菌B22在蓝色LED下产生的蛋白酶可用于工业应用。