A high-performance liquid chromatographic (HPLC) method and a cyclodextrin-modified micellar electrokinetic chromatographic (CD-MEKC) method were developed to separate and determine oleanolic acid (OA) and ursolic acid (UA) in Prunella vulgaris. HPLC separations were carried out on a Hedera ODS C18 column with methanol -H₂O- acetic acid (85:15:0.3, v/v/v) as mobile phase at a flow-rate of 0.8 ml min⁻¹. CD-MEKC analysis was performed on a CL1030 capillary electrophoresis system with a 6% (v/v) methanol solution (pH = 9.0) containing 10 mM disodium tetraborate, 10 mM sodium dihydrogen phosphate, 50 mM sodium dodecylsulfate (SDS), 15 mM 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) as background electrolyte. The analytical results of HPLC and CD-MEKC were compared with each other. CD-MEKC has better analytical efficiency for two components, and the analytical time (15 min) was shorter than that of HPLC (35 min).

中文翻译：

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高效液相色谱和环糊精修饰的胶束电动色谱法分离夏枯草中齐墩果酸和熊果酸的比较研究

开发了一种高效液相色谱（HPLC）方法和环糊精修饰的胶束电动色谱（CD-MEKC）方法，以分离和测定寻常型夏枯草中的齐墩果酸（OA）和熊果酸（UA）。HPLC分离在Hedera ODS C18色谱柱上进行，甲醇-H ₂ O-乙酸（85：15：0.3，v / v / v）作为流动相，流速为0.8 ml min ^-1。CD-MEKC分析是在CL1030毛细管电泳系统上进行的，其中使用6％（v / v）甲醇溶液（pH = 9.0）包含10 mM四硼酸钠，10 mM磷酸二氢钠，50 mM十二烷基硫酸钠（SDS），15 mM 2-羟丙基-β-环糊精（2-HP-β-CD）作为背景电解质。将HPLC和CD-MEKC的分析结果进行了比较。CD-MEKC对两种成分的分析效率更高，并且分析时间（15分钟）比HPLC（35分钟）短。