Natural circulating antibodies (NAbs) to endogenous regulators have shown to be potential biomarkers in medicine. Due to the lack of reliable assays, only few of them have been well studied. To employ NAbs as biomarkers, an evaluation of changes over the course of a treatment is required. This paper describes our work to analyze the dynamics of NAbs titer to interferon-gamma (IFN-γ) among healthy children of different age and in patients with varicella infection receiving an antiviral drug Anaferon for children (AC, the API are highly diluted antibodies to IFN-γ) in comparison with placebo, and to correlate the findings with the treatment results. IFN-γ plays an essential role during varicella infection, and this fact causes the consequent increase of NAbs to IFN-γ level. The mean anti-IFN-γ NAbs level in the healthy volunteer group was 101 × 103 U/ml (age: 0-15 years), which was significantly lower than the mean pre-treatment value in patients with varicella infection 167 × 103 U/ml (age: 3-17 years). In the AC group, the NAbs level observed on days 5 and 10 decreased significantly to a level of 154 × 103 U/ml, whereas in the placebo group it continued to rise in a time-dependent manner reaching 229 × 103 U/ml on day 10. Our findings suggest that treatment with AC is characterized by "normalization" of the anti-IFN-γ NAbs levels in patients with varicella infection.

中文翻译：

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用儿童抗病毒药物Anaferon治疗的水痘感染中自然抵抗IFN-γ的自身抗体水平：一项先导研究。

内源性调节剂的天然循环抗体（NAbs）已被证明是医学上潜在的生物标志物。由于缺乏可靠的检测方法，只有很少的方法得到了很好的研究。要将NAbs用作生物标记，需要评估治疗过程中的变化。本文介绍了我们的工作，以分析不同年龄的健康儿童以及接受抗病毒药物儿童Anaferon的水痘感染患者中NAb滴度对干扰素-γ（IFN-γ）的动力学（AC，API是针对IFN-γ）与安慰剂比较，并将发现与治疗结果相关联。IFN-γ在水痘感染期间起着至关重要的作用，这一事实导致了NAb随之升高至IFN-γ水平。健康志愿者组的平均抗IFN-γNAbs水平为101×103 U / ml（年龄：0-15岁），显着低于水痘感染患者的平均治疗前值167×103 U / ml。 / ml（年龄：3-17岁）。在AC组中，在第5天和第10天观察到的NAbs水平显着下降至154×103 U / ml，而在安慰剂组中，NAbs持续呈时间依赖性地上升，在第7天达到229×103 U / ml。第10天。我们的发现表明，水痘感染患者中AC治疗的特点是抗IFN-γNAbs水平“正常化”。