Insect diapause is associated with physiological dormancy, extension of life span and an altered developmental programme. Reactive oxygen species, oxidative damaged products and antioxidants are being increasingly linked to such processes in insects. Tropical tasar silkworm, Antheraea mylitta Drury (Lepidoptera:Saturniidae), has an impressive pupal diapause and exhibits a distinct pattern of development in nondiapausing (NDP) pupa compared to that of diapausing (DP) pupa. In this study, we have analysed the amount of hydrogen peroxide (H2O2) and oxidative damaged products along with the levels of antioxidant protection in the hemolymph (HL) and fat body (FB) tissues of developing pupa of A. mylitta from DP and NDP generations having identical chronological age but distinct physiological status. Signature of oxidative predominance was clearly marked in the tissues of diapause-destined pupa as evident from a higher level of lipid peroxidation and protein carbonyl content, despite a lower level of H2O2 in the said group. Specific activity of catalase, exclusively observed in the FB was significantly higher in diapause-destined pupae. Total superoxide dismutase activity was found to be higher in both tissue types of diapause-destined pupae compared to their NDP counterpart. In contrast, pupae committed for diapause displayed less activity for glutathione S-transferase than the NDP group. Reduced glutathione exhibited tissue specificity being higher in the FB of NDP pupae. Findings of this study suggest that low H2O2 pool with a strategic antioxidant protection is associated with induction of dormancy and enhanced oxidative resistance during an extended lifespan.

中文翻译：

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低ħ ₂ ö ₂和增强在蚕的滞育注定蛹耐氧化性，柞蚕（鳞翅目：蚕蛾）建议在休眠和寿命延长的可能参与

昆虫滞育与生理学休眠，寿命延长和发育计划改变有关。活性氧，氧化破坏产物和抗氧化剂正越来越多地与昆虫中的此类过程联系在一起。热带tasar蚕Antheraea mylitta Drury（鳞翅目：Saturniidae）具有令人印象深刻的p滞育作用，与滞育（DP）育种相比，非滞育（NDP）育种表现出独特的发育模式。在这项研究中，我们分析了从DP和NDP发育的A. mylitta的血淋巴（HL）和脂肪体（FB）组织中过氧化氢（H2O2）和氧化损伤产物的量以及抗氧化保护的水平年龄相同但生理状态不同的几代人。尽管在该组中H 2 O 2的水平较低，但脂质滞留和蛋白质羰基含量的较高水平清楚地表明了滞育性-的组织中氧化优势的特征。在滞育目的的p中，仅在FB中观察到的过氧化氢酶的比活性显着较高。发现滞育性p的两种组织类型中的总超氧化物歧化酶活性均高于其NDP对应物。相反，定为滞育的p显示出比NDP组少的谷胱甘肽S-转移酶活性。还原型谷胱甘肽在NDP up的FB中表现出更高的组织特异性。