Fluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated DNA using fluorescent nucleic acid probes. A couple of functional fluorescent nucleic acid probes developed by our laboratory are introduced and the examples of their application to fluorescence imaging of intracellular nucleic acids are described.

中文翻译：

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用于细胞内核酸显微分析的下一代荧光核酸探针

核酸的荧光成像是了解基因表达和由此产生的细胞功能变化所必需的一项非常重要的技术。这篇小型综述侧重于使用荧光核酸探针对细胞内 RNA 和甲基化 DNA 进行序列特异性荧光成像。介绍了我们实验室开发的几种功能性荧光核酸探针，并介绍了它们在细胞内核酸荧光成像中的应用实例。