The present study focused upon comprehensive analysis of the expression of stress-responsive genes at different time points in two indica rice varieties, IR-64 (moderately salt-sensitive) and Nonabokra (salt-tolerant), under salinity, dehydration and cold, and exogenous ABA treatment. The massively parallel signature sequencing and publicly-available microarray data indicated the physiological role of the concerned genes in different tissues. Gene expression through semi-quantitative reverse transcriptase-polymerase chain reaction followed by factorial analyses of variance identified that members of the antioxidant genes (OsCAT, OsSOD, OsGR2 and OsAPX8) showed early response and higher expression under different abiotic stresses in IR-64. The TFs and LEA family (TRAB1 and Osem), osmolyte (OsBADH1, OsP5CS) and polyamine (SAMDC) metabolic genes were more up regulated in the salt-tolerant cultivar, whereas the proline (ProDH) and polyamine (DAO and PAO) catabolic genes were significantly up regulated in the salt-sensitive one. The higher accumulation of TRAB1 transcript and protein in the tolerant cultivar during various stresses suggested it as an important component in stress signaling pathway, which participates in mediating cross-talk signaling between diverse metabolic pathways to withstand various forms of abiotic stress. Heat map data further corroborated differential transcript abundance in the two varieties, coinciding with the transcript profiling data. We envisage that the identified genes in this study, linked with tolerance against various abiotic stress factors, are the chief candidates involved in regulating the complexity of multiple stress responses in rice. The finding of the candidate genes in this study would be helpful in generating multiple abiotic stress tolerance in rice.

中文翻译：

---

两种in稻品种中脱落酸诱导基因响应不同非生物干扰的转录产物分析

本研究着重于盐分，脱水和寒冷条件下，两个two稻品种IR-64（中度盐敏感）和Nonabokra（耐盐）在不同时间点的胁迫响应基因的表达的综合分析，以及外源ABA治疗。大量的平行签名测序和公开可用的微阵列数据表明了相关基因在不同组织中的生理作用。通过半定量逆转录酶-聚合酶链反应进行基因表达，然后进行方差分析，确定了抗氧化剂基因的成员（OsCAT，OsSOD，OsGR2和OsAPX8）在IR-64中在不同的非生物胁迫下显示出早期反应和更高的表达。耐盐品种的TF和LEA家族（TRAB1和Osem），渗透液（OsBADH1，OsP5CS）和多胺（SAMDC）代谢基因上调，而脯氨酸（ProDH）和多胺（DAO和PAO）在盐敏感的一种中，分解代谢基因显着上调。在各种胁迫下，耐性品种中TRAB1转录和蛋白的较高积累表明它是胁迫信号通路中的重要组成部分，其参与介导不同代谢途径之间的串扰信号，以抵抗各种形式的非生物胁迫。热图数据进一步证实了两个品种的差异转录本丰度，与转录本谱图数据相吻合。我们设想，这项研究中鉴定出的基因与对各种非生物胁迫因素的耐受性相关，是参与调节水稻多重胁迫反应的复杂性的主要候选对象。在这项研究中候选基因的发现将有助于在水稻中产生多种非生物胁迫耐受性。