A simple microsphere-based mold to rapidly fabricate microwell arrays for multisize 3D tumor culture.,Biotechnology and Bioengineering

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A simple microsphere-based mold to rapidly fabricate microwell arrays for multisize 3D tumor culture.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-01-27 , DOI: 10.1002/bit.27257
Zixiu Li ₁ , Xiaofang Guo ₁ , Lili Sun ₁ , Juan Xu ₁ , Wenming Liu ₂ , Tianbao Li ₁ , Jinyi Wang ₁

Affiliation

Three‐dimensional (3D) tumor has been considered as the best in vitro model for cancer research. In recent years, various methods have been developed to controllable prepare multisize 3D tumors. Nonetheless, reported technologies are still problematic and difficult to produce 3D tumors with highly uniform size and cell content. Here, a novel and simple microsphere‐based mold approach is proposed to rapidly fabricate spherical microwell arrays for multisize 3D tumors formation, culture, and recovery. Larger amounts of HepG2 3D tumors with excellent quality and uniformity can be efficiently generated using this method. In addition, the tumor size can also be simply controlled by adjusting the diameter of the microwell arrays. All experimental results indicated that the proposed method offers a promising platform to generate and recover highly controlled multisize 3D tumors for various cell‐based biomedical research.

中文翻译：

一个基于微球的简单模具，可快速制造用于多尺寸3D肿瘤培养的微孔阵列。

三维（3D）肿瘤被认为是癌症研究的最佳体外模型。近年来，已经开发出各种方法来可控地制备多尺寸3D肿瘤。尽管如此，已报道的技术仍然存在问题，并且难以产生尺寸和细胞含量高度统一的3D肿瘤。在这里，提出了一种新颖且简单的基于微球的模具方法来快速制造球形微孔阵列，以用于多种尺寸的3D肿瘤的形成，培养和恢复。使用此方法可以有效地生成大量具有优良质量和均匀性的HepG2 3D肿瘤。另外，还可以通过调节微孔阵列的直径来简单地控制肿瘤大小。

更新日期：2020-03-09

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