DNA origami is a powerful tool in nanotechnology that can be used to construct arbitrary structures for several nanoengineering applications. Generally, the more complex and sophisticated the construction, the greater is the number of origamis and connection strands that will be needed. Therefore, developing an effective and low-cost method for multiform DNA architecture is important in nanoengineering. Here, we adopted an oblique linking strategy to connect cross-shaped DNA origami with a controlled curing angle. The size of the DNA rings ranged from four blocks of approximately 200 nm to eleven blocks of c.a. 600 nm. We observed that the minimum size of the DNA ring structure was limited by the width of a single block. The largest rings were negatively affected by thermodynamic randomness, and thus, DNA rings consisting of more than eleven blocks were not observed. This strategy facilitates the generation of various DNA origami rings, whose size can be controlled by adjusting the length of the connection strands.

中文翻译：

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调整连接链以形成尺寸可控的 DNA 折纸环


DNA折纸是纳米技术中的一种强大工具，可用于为多种纳米工程应用构建任意结构。一般来说，结构越复杂和精细，所需的折纸和连接线的数量就越多。因此，开发一种有效且低成本的多形态DNA结构方法在纳米工程中具有重要意义。在这里，我们采用了倾斜连接策略，以受控的固化角度连接十字形 DNA 折纸。 DNA环的大小范围从4个约200 nm的块到11个约600 nm的块。我们观察到 DNA 环结构的最小尺寸受到单个块宽度的限制。最大的环受到热力学随机性的负面影响，因此，没有观察到由超过 11 个块组成的 DNA 环。这种策略有利于各种DNA折纸环的生成，其大小可以通过调整连接链的长度来控制。