Abstract The glutaraldehyde fixation method for fixing tissues is attractive for its ease of use and straightforward surface chemistry. We investigated the effect of glutaraldehyde-induced microtubule immobilization on kinesin-driven cargo transport along microtubules and found that at low glutaraldehyde concentrations, the microtubule–kinesin interaction remains unperturbed. Such findings may facilitate the application of the glutaraldehyde fixation method for many in vitro studies aiming to build nanodevices powered by the microtubule–motor protein interaction. The graphical abstract shows that the kinesin-driven quantum dot transport along microtubule immobilized on a substrate using glutaraldehyde concentration ≤0.10% (v/v) remain unaltered, whereas, at higher glutaraldehyde concentration, >0.10% (v/v), the quantum dot transportation is slowed down.

中文翻译：

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戊二醛固定微管对驱动蛋白驱动的货物运输的影响

摘要 用于固定组织的戊二醛固定方法因其易用性和简单的表面化学而具有吸引力。我们研究了戊二醛诱导的微管固定对驱动蛋白驱动的货物沿微管运输的影响，发现在低戊二醛浓度下，微管-驱动蛋白相互作用保持不变。这些发现可能有助于将戊二醛固定方法应用于许多旨在构建由微管-运动蛋白相互作用驱动的纳米装置的体外研究。图形摘要显示驱动蛋白驱动的量子点沿固定在基质上的微管传输，使用戊二醛浓度≤0.10% (v/v) 保持不变，而在较高的戊二醛浓度下，> 0.10% (v/v)，