The circadian pacemaker in plants is a hierarchical multioscillator system that directs and maintains a 24-hr oscillation required for organism homeostasis and environmental fitness. Molecular clockwork within individual tissues and organs acts cell autonomously, showing differences in circadian expression of core oscillators and their target genes; there are functional dominance and coupling in the complex regulatory network. However, molecular characteristics of organ-specific clocks are still unknown. Here, we showed the detached shoot and root possess dynamic circadian protein-protein interactions between clock core components, periodicity in organs exhibits a difference. The period length difference between shoot and root was not remarkable in prr7-3 and prr7-3 prr9-1 mutants. In addition, the phase transition curve indicated that shoot and root clock respond differently to the resetting cues of ambient temperature. PRR9 and PRR7 compensate circadian period between 22°C and 28°C in shoot, not in root. The circadian rhythms of PRR9 or PRR7 transcript accumulation showed no difference at 22°C and 28°C in shoot, but differences were observed in root. In summary, our results reveal the specificity of dynamic circadian protein-protein interactions in organ-autonomous clocks and the critical roles of PRR9 and PRR7 in mechanisms regulating temperature compensation in aerial shoot system.

中文翻译：

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拟南芥昼夜节律振荡器的器官自主表达的分子研究。

植物中的昼夜节律起搏器是一种分层的多振子系统，可指导并维持生物体稳态和环境适应性所需的24小时振荡。各个组织和器官内的分子发条会自主作用于细胞，显示出核心振荡器及其靶基因的昼夜节律表达存在差异。复杂的监管网络中存在着功能上的主导和耦合。但是，器官特异性时钟的分子特征仍然未知。在这里，我们显示了离生的茎和根在时钟核心组件之间具有动态的昼夜节律蛋白相互作用，器官中的周期性表现出差异。在prr7-3和prr7-3 prr9-1突变体中，芽和根之间的周期长度差异不明显。此外，相变曲线表明枝条和根时钟对环境温度的复位信号的响应不同。PRR9和PRR7可以补偿枝条（而不是根系）在22°C至28°C之间的昼夜节律。PRR9或PRR7转录本积累的昼夜节律在芽中在22°C和28°C时无差异，但在根部观察到差异。总之，我们的研究结果揭示了器官自主钟中动态昼夜节律蛋白质相互作用的特异性，以及PRR9和PRR7在调控空中射击系统温度补偿的机制中的关键作用。PRR9或PRR7转录本积累的昼夜节律在芽中在22°C和28°C时无差异，但在根部观察到差异。总之，我们的研究结果揭示了器官自主钟中动态昼夜节律蛋白质相互作用的特异性，以及PRR9和PRR7在调控空中射击系统温度补偿的机制中的关键作用。PRR9或PRR7转录本积累的昼夜节律在芽中在22°C和28°C时无差异，但在根部观察到差异。总之，我们的研究结果揭示了器官自主钟中动态昼夜节律蛋白质相互作用的特异性，以及PRR9和PRR7在调控空中射击系统温度补偿的机制中的关键作用。