Stereotaxic endoscopy for the ocular imaging of awake, freely moving animal models.,Journal of Biophotonics

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Stereotaxic endoscopy for the ocular imaging of awake, freely moving animal models.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-02-16 , DOI: 10.1002/jbio.201960188
Bjorn Paulson ₁ , Sangwook Lee ₂ , Miyeon Jue ₁ , Kyungsung Lee ₁ , Sanghwa Lee ₁ , Guk Bae Kim ₃ , Youngjin Moon _{1,

2} , Joo Yong Lee ₄ , Namkug Kim ₂ , Jun Ki Kim _{1,

2}

Affiliation

Stereotaxic instruments are increasingly used in research animals for the study of disease, but typically require restraints and anesthetic procedures. A stereotaxic head mount that enables imaging of the anterior chamber of the eye in alert and freely mobile mice is presented in this study. The head mount is fitted based on computed tomography scans and manufactured using 3D printing. The system is placed noninvasively using temporal mount bars and a snout mount, without breaking the skin or risking suffocation, while an instrument channel stabilizes the ocular probes. With a flexible micro‐endoscopic probe and a confocal scanning laser microscopy system, <20 μm resolution is achieved in vivo with a field of view of nearly 1 mm. Discomfort is minimal, and further adaptations for minimally invasive neuroscience, optogenetics and auditory studies are possible.

中文翻译：

立体定向内窥镜用于清醒，自由移动的动物模型的眼部成像。

立体定向仪器越来越多地用于研究动物的疾病研究中，但通常需要进行约束和麻醉程序。这项研究提出了一种立体定位的头部固定装置，可以使机敏和自由移动的小鼠的眼前房成像。头枕基于计算机断层扫描技术进行安装，并使用3D打印进行制造。该系统使用临时安装杆和鼻子安装架进行非侵入式放置，而不会破坏皮肤或造成窒息危险，同时仪器通道可稳定眼探针。借助柔性微内窥镜探头和共聚焦扫描激光显微镜系统，体内分辨率可达到<20μm视场近1毫米。不适感极小，并且可能会进一步适应微创神经科学，光遗传学和听觉研究。

更新日期：2020-02-16

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