BACKGROUND Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive adhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. RESULTS Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. CONCLUSION The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.

中文翻译：

---

乳酸菌的作用。空肠弯曲杆菌在鸡和猪小肠上皮细胞系中的粘附、侵袭和易位的影响。


背景弯曲杆菌属。是细菌性食源性腹泻病的主要原因。这主要是由于肉制品在加工过程中受到污染造成的。对于感染，弯曲杆菌属。必须粘附在粘液层的上皮细胞上，在胃肠道的条件下生存，并在宿主的肠道中定殖。添加益生菌可能会促进与上皮细胞的竞争性粘​​附，从而减少空肠弯曲杆菌的定植。乳酸菌的作用。研究了（PCS20、PCS22、PCS25、LGG、PCK9）对猪（PSI cl.1）和鸡（B1OXI）小肠细胞系以及猪肠细胞（CLAB）中空肠弯曲菌粘附、侵袭和易位的影响。结果 总体而言，在 PSI cl.1 和 CLAB 单层细胞的竞争性粘​​附测定中，添加乳杆菌属。减少了空肠弯曲菌对细胞表面的粘附，并对空肠弯曲菌的入侵产生了负面影响。有趣的是，乳酸菌属。在三维功能性 PSI cl.1 和 B1OXI 细胞模型中，空肠弯曲菌粘附显着受损。此外，当与益生菌共孵育时，空肠弯曲菌不会跨过 PSI cl.1 和 B1OXI 细胞单层。在选定的益生菌中，鼠李糖乳杆菌 LGG 是在共培养条件下最显着降低空肠弯曲菌粘附功效的菌株。结论 添加乳酸菌。在牲畜营养中添加饲料添加剂可能是一种有效的新策略，其目标是弯曲杆菌粘附在上皮细胞上，从而防止定植，减少传播，并最终降低人类弯曲杆菌病的发病率。