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Phenotypic antimicrobial resistance in Escherichia coli strains isolated from swine husbandries in North Western Germany - temporal patterns in samples from laboratory practice from 2006 to 2017.
BMC Veterinary Research ( IF 2.3 ) Pub Date : 2020-02-03 , DOI: 10.1186/s12917-020-2268-z
C Moennighoff 1 , N Thomas 2 , F Nienhaus 1 , M Hartmann 2 , A Menrath 1 , J Merkel 3 , H Detlefsen 1 , L Kreienbrock 2 , I Hennig-Pauka 1
Affiliation  

BACKGROUND Since 2011, antibiotic usage has decreased continuously in livestock in Germany. Whether this is accompanied by a reduction in bacterial antimicrobial resistance has not been proven so far. In this study 3054 Escherichia coli (E. coli) isolates from pigs which had suffered from disease on 2161 farms in North Western Germany were evaluated retrospectively from 2006 to 2017 for trends in their antimicrobial resistance pattern. Data were substantially related to the "pre-reduction period" and were therefore suggested as a basis for this task. Minimal inhibitory concentrations for selected antimicrobial substances were evaluated for E. coli strains isolated from different organs of diseased swine sampled for routine diagnostic. In total, 81% of E. coli were isolated from faeces or the gastrointestinal tract, 11% from the genito-urinary tract and 8% from other organs. Susceptibility testing and classification of isolates in accordance with clinical cut-offs followed the Clinical and Laboratory Standards Institute (CLSI). If no clinical cut-offs were available for the respective combination of species, substance and organ, other published clinical cut-offs were used. RESULTS Differences in susceptibility patterns between isolates from the gastrointestinal and genito-urinary tract were found for most substances. Isolates from the genito-urinary tract were less frequently resistant to ampicillin, apramycin, colistin, neomycin, spectinomycin and tetracycline and more frequently resistant to enrofloxacin and florfenicol. A multifactorial logistic regression model revealed time-dependent decreases in frequency of resistant isolates for neomycin, spectinomycin and tetracycline. For colistin, the highest percentage of resistant isolates with 16.0% was found in 2015 followed by a decrease to the level of 2009-2010 in 2017. A decrease in frequencies of ampicillin-resistant isolates was dependent on the age-group and time period. Irrespective of the year, less than 15% E. coli isolates were resistant to apramycin, cephalosporins, colistin, enrofloxacin, florfenicol, gentamicin and neomycin. CONCLUSION An overall time-dependent decrease in the percentage of resistant E. coli isolates was found for some substances. These data from diseased animals indicate an impact of a general reduction in antibiotic usage on development of bacterial antimicrobial resistance in the field and can support the decision-making of swine practitioners for treatment options in swine.

中文翻译:

从德国西北部养猪场分离的大肠杆菌菌株的表型抗菌素耐药性-2006年至2017年实验室实践中样品的时间模式。

背景技术自2011年以来,德国牲畜的抗生素使用量持续下降。到目前为止,是否伴随细菌抗药性的降低还没有得到证实。在这项研究中,对2006年至2017年间从德国西北部2161个农场患病的猪中分离出的3054株大肠杆菌进行了回顾性评估,以了解其抗菌素耐药性趋势。数据与“还原前期”基本相关,因此建议将其作为该任务的基础。对于从常规疾病进行诊断的患病猪不同器官中分离的大肠杆菌菌株,评估了所选抗菌物质的最低抑菌浓度。总共有81%的大肠杆菌是从粪便或胃肠道中分离出来的,生殖泌尿道占11%,其他器官占8%。遵循临床和实验室标准对药敏试验和分离株分类遵循临床和实验室标准协会(CLSI)。如果没有针对物种,物质和器官各自组合的临床临界值,则使用其他已公布的临床临界值。结果对于大多数物质,胃肠道和生殖泌尿道分离株之间的药敏模式存在差异。来自生殖泌尿道的分离株对氨苄青霉素,阿普霉素,粘菌素,新霉素,壮观霉素和四环素的抵抗力较弱,对恩诺沙星和氟苯尼考的​​抵抗力更强。多因素Logistic回归模型显示,新霉素耐药菌的频率随时间的降低,大观霉素和四环素。对于大肠菌素,2015年发现耐药菌株的最高百分比,为16.0%,随后在2017-2017年下降到2009-2010年的水平。氨苄青霉素耐药菌株的频率减少取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。对于大肠菌素,2015年发现耐药菌株的最高百分比,为16.0%,随后在2017-2017年下降到2009-2010年的水平。氨苄青霉素耐药菌株的频率减少取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。对于大肠菌素,2015年发现耐药菌株的最高百分比,为16.0%,随后在2017-2017年下降到2009-2010年的水平。氨苄青霉素耐药菌株的频率减少取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。2015年发现0%,随后在2017-2009年下降到2009-2010年水平。耐氨苄青霉素分离株的频率下降取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。2015年发现0%,随后在2017-2009年下降到2009-2010年水平。耐氨苄青霉素分离株的频率下降取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。耐氨苄青霉素分离株的频率降低取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。耐氨苄青霉素分离株的频率降低取决于年龄组和时间段。不论年份如何,只有不到15%的大肠杆菌分离株对阿普霉素,头孢菌素,粘菌素,恩诺沙星,氟苯尼考,庆大霉素和新霉素耐药。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。结论发现某些物质的耐药大肠杆菌分离物百分比总体上呈时间依赖性降低。来自患病动物的这些数据表明,抗生素用量的普遍减少对该领域细菌抗菌素耐药性的发展会产生影响,并且可以支持养猪从业者做出猪治疗选择的决策。
更新日期:2020-02-04
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