This review summarizes the main applications of different sections and some improved sectioning methods in investigating the microstructure and histochemistry of cereal kernels. Thick sections of developing kernels prepared by free-hand and sliding microtome-aided sectioning method can be employed to elucidate tissue anatomy and histochemistry. The thin sections of mature kernels prepared by ultramicrotome-aided sectioning method can exhibit the micromorphology of starch granules when stained with iodine solution. The paraffin sections of developing kernels can exhibit the tissue anatomy of kernel, the accumulation of storage substances, and the location of protein and gene transcripts with immunohistochemistry and in situ hybridization techniques. The semithin resin sections can clearly exhibit the morphology of cells, starch granules, and protein bodies in kernel, but the sections prepared with different resins have various advantages and disadvantages for research investigating the morphology and histochemistry of cereal kernels. The improved methods of free-hand sectioning and ultramicrotome-aided sectioning of mature kernels are suitable for investigating the morphology of starch granules in a large number of samples in a short time. The modified method for preparing resin sections of whole kernels can be employed to determine the morphology and distribution of cells, starch granules, and storage protein in mature, developing, germinated, and cooked kernels in situ. This review could help researchers choose appropriate sections for investigating the microstructure and histochemistry of cereal kernels according to their study objectives.

中文翻译：

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在研究谷物仁的微观结构和组织化学中，不同部分的综合比较和应用。

这篇综述总结了不同部分的主要应用以及一些改进的切片方法在研究谷粒的微观结构和组织化学中的应用。通过徒手和滑动切片机辅助切片方法制备的发育核的厚切片可用于阐明组织解剖结构和组织化学。通过超薄切片机切片法制备的成熟籽粒的薄切片在用碘溶液染色时可以表现出淀粉颗粒的微观形态。通过免疫组织化学和原位杂交技术，发育中的核仁的石蜡切片可以显示出核仁的组织解剖结构，储存物质的积累以及蛋白质和基因转录物的位置。半薄的树脂切片可以清楚地显示出细胞，淀粉颗粒，和谷物中的蛋白质体，但是用不同树脂制备的切片对研究谷物仁的形态和组织化学的研究有各种优点和缺点。徒手切片和超薄切片机切片的改进方法适合于在短时间内研究大量样品中淀粉颗粒的形态。改进的全仁树脂切片制备方法可用于确定成熟，发育，发芽和煮熟的仁中细胞，淀粉颗粒和贮藏蛋白的形态和分布。这项综述可以帮助研究人员根据他们的研究目标选择合适的部分研究谷物仁的微观结构和组织化学。但是，用不同树脂制备的切片对研究谷粒的形态和组织化学的研究有各种优点和缺点。徒手切片和超薄切片机切片的改进方法适合于在短时间内研究大量样品中淀粉颗粒的形态。改进的全仁树脂切片制备方法可用于确定成熟，发育，发芽和煮熟的仁中细胞，淀粉颗粒和贮藏蛋白的形态和分布。这项综述可以帮助研究人员根据他们的研究目标选择合适的部分研究谷物仁的微观结构和组织化学。但是，用不同树脂制备的切片对研究谷粒的形态和组织化学的研究有各种优点和缺点。徒手切片和超薄切片机切片的改进方法适合于在短时间内研究大量样品中淀粉颗粒的形态。改进的全仁树脂切片制备方法可用于确定成熟，发育，发芽和煮熟的仁中细胞，淀粉颗粒和贮藏蛋白的形态和分布。这项综述可以帮助研究人员根据他们的研究目标选择合适的部分研究谷物仁的微观结构和组织化学。