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Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-02-18 , DOI: 10.1021/acssensors.9b02205 Ping Liu 1, 2 , Bowei Li 2, 3 , Longwen Fu 2, 3 , Yan Huang 2, 4 , Mingsan Man 2 , Ji Qi 2 , Xiyan Sun 2, 3 , Qi Kang 1 , Dazhong Shen 1 , Lingxin Chen 2, 3, 4
ACS Sensors ( IF 8.2 ) Pub Date : 2020-02-18 , DOI: 10.1021/acssensors.9b02205 Ping Liu 1, 2 , Bowei Li 2, 3 , Longwen Fu 2, 3 , Yan Huang 2, 4 , Mingsan Man 2 , Ji Qi 2 , Xiyan Sun 2, 3 , Qi Kang 1 , Dazhong Shen 1 , Lingxin Chen 2, 3, 4
Affiliation
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In this paper, we first proposed a novel hybrid three-dimensional (3D) printed and paper-based microfluidic platform and applied it for investigating the cell's apoptosis and intracellular cross-talk. The fabrication of a 3D-printed microfluidic chip is much easier than polydimethylsiloxane (PDMS) chip and can be applied in many common labs without soft lithogrophy fabrication equipment. Moreover, 3D printing can be perfectly combined with paper-based chips that can provide 3D scaffold for cell culture and analysis. In addition, these paper chips are disposable after use, greatly reducing the experimental cost. We integrated "Christmas Tree" structure with the top layer of the 3D-printed microfluidic chip to generate a continuous concentration gradient, and the bottom layer contained paper-based chips as cell culture area. The two-layer structure allows the concentration gradient forming layer to be separated from the cell culture layer, which can simplify the planting of cells in the microfluidic chip and make sure the cells stay in the culture chambers and don't clog the microfluidic channels. Applying this hybrid platform, we examined the effect of H2S on cancer cells. Continuous exposure to a low concentration of H2S inhibited cancer cell SMMC-7721 proliferation by inducing cell apoptosis. We also found that two gaseous molecules H2S and NO have cross-talk in cancer cells; they formed bioactive intermediate polysulfides in cancer cells. It is expected that this novel hybrid 3D-printed and paper-based microfluidic platform will have widespread application prospects in cell investigation.
中文翻译：
混合三维打印纸基微流控平台，用于研究细胞的凋亡和细胞内交叉谈话。
在本文中，我们首先提出了一种新型的混合式三维（3D）打印和纸基微流体平台，并将其用于研究细胞的凋亡和细胞内串扰。3D打印的微流控芯片的制造比聚二甲基硅氧烷（PDMS）芯片容易得多，并且可以在许多普通实验室中使用，而无需使用软石印术制造设备。此外，3D打印可以与纸基芯片完美结合，后者可以为细胞培养和分析提供3D支架。另外，这些纸屑在使用后是一次性的，大大降低了实验成本。我们将“圣诞树”结构与3D打印的微流控芯片的顶层集成在一起，以生成连续的浓度梯度，并且底层包含纸基芯片作为细胞培养区域。两层结构允许浓度梯度形成层与细胞培养层分离，这可以简化细胞在微流控芯片中的种植，并确保细胞留在培养室中并且不会阻塞微流控通道。应用此混合平台，我们检查了H2S对癌细胞的影响。持续暴露于低浓度的H2S中会诱导细胞凋亡，从而抑制癌细胞SMMC-7721的增殖。我们还发现，两个气态分子H2S和NO在癌细胞中具有串扰作用。他们在癌细胞中形成了具有生物活性的中间体多硫化物。可以预期，这种新颖的混合3D打印纸质微流体平台将在细胞研究中具有广泛的应用前景。
更新日期：2020-02-18
中文翻译：
混合三维打印纸基微流控平台，用于研究细胞的凋亡和细胞内交叉谈话。
在本文中，我们首先提出了一种新型的混合式三维（3D）打印和纸基微流体平台，并将其用于研究细胞的凋亡和细胞内串扰。3D打印的微流控芯片的制造比聚二甲基硅氧烷（PDMS）芯片容易得多，并且可以在许多普通实验室中使用，而无需使用软石印术制造设备。此外，3D打印可以与纸基芯片完美结合，后者可以为细胞培养和分析提供3D支架。另外，这些纸屑在使用后是一次性的，大大降低了实验成本。我们将“圣诞树”结构与3D打印的微流控芯片的顶层集成在一起，以生成连续的浓度梯度，并且底层包含纸基芯片作为细胞培养区域。两层结构允许浓度梯度形成层与细胞培养层分离，这可以简化细胞在微流控芯片中的种植，并确保细胞留在培养室中并且不会阻塞微流控通道。应用此混合平台，我们检查了H2S对癌细胞的影响。持续暴露于低浓度的H2S中会诱导细胞凋亡，从而抑制癌细胞SMMC-7721的增殖。我们还发现，两个气态分子H2S和NO在癌细胞中具有串扰作用。他们在癌细胞中形成了具有生物活性的中间体多硫化物。可以预期，这种新颖的混合3D打印纸质微流体平台将在细胞研究中具有广泛的应用前景。
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