This study aimed to evaluate in vivo the effect of laser photobiomodulation associated with a repair cement (MTA Repair HP™) on the process of bone repair in the femur of rats, through histological and histomorphometric assays. Forty Wistar albino rats were randomly divided into four groups, with two periods of euthanasia - 15 and 21 days (n = 5 per period). Under general anesthesia, a bone defect was made in the left femur of each animal. In the LS (Laser) group, the defect was irradiated following the parameters: λ = 808 nm, P = 100 mW, ED = 80 J/cm2 per point, 22 s per point, E = 2.2 J per point. In the LM (MTA Repair HP™ + Laser) group, the defect was filled with MTA Repair HP™ and irradiated with laser in the same protocol of the LS group. In the MH (MTA Repair HP™) group, the defect was filled with MTA Repair HP™ without irradiation. In the CTR (Control) group, the bone defect received no treatment. At 15 days, the mean index of bone neoformation in the defect area was significantly lower in the CTR group as compared to the MH, LS, and LM groups. At 21 days, the LM group presented significantly greater bone neoformation than the MH group, without significant difference between LS and LM. Laser photobiomodulation therapy is promising as an adjuvant in the bone repair process, especially when associated with the use of biomaterials.

中文翻译：

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激光光调制与生物陶瓷胶结物对大鼠股骨骨组织修复的影响。

这项研究旨在通过组织学和组织形态计量学方法评估与修复水泥（MTA Repair HP™）相关的激光光生物调节对大鼠股骨骨修复过程的影响。将40只Wistar白化病大鼠随机分为四组，分为两个时期的安乐死-15天和21天（每个时期n = 5）。在全身麻醉下，在每只动物的左股骨中形成骨缺损。在LS（激光）组中，按照以下参数辐照缺陷：λ= 808 nm，P = 100 mW，ED =每点80 J / cm2，每点22 s，E = 2.2J。在LM（MTA修复HP™+激光）组中，用MTA修复HP™填充缺损，并按照与LS组相同的方案用激光照射。在MH（MTA Repair HP™）组中，未经放射的MTA Repair HP™填补了缺损。在CTR（对照组）组中，骨缺损未得到治疗。在第15天时，与MH，LS和LM组相比，CTR组的缺损区域骨新形成的平均指数显着降低。在21天时，LM组比MH组表现出明显更大的骨新形成，而LS和LM之间无显着差异。激光光生物调节疗法有望在骨修复过程中作为佐剂，特别是与生物材料的使用相关时。