Abstract Histamine plays a key role in inflammatory responses via increasing chemokine and adhesion molecule productions and augmenting vascular permeability in endothelial cells. Rhodomyrtus tomentosa has been found as a rich source of structurally diverse and biologically active metabolites. In this study, the role of phenolic compound from Rhodomyrtus tomentosa (Aiton) Hassk fruits in down-regulation of histamine-induced EA.hy926 endothelial cell activation was investigated. Herein, myricetin was successfully isolated from R. tomentosa fruits by HPLC and its characterization was identified by mass spectrometer and nuclear magnetic resonance spectroscopy. It was revealed that myricetin was effective in suppression of IL-8 and MCP-1 productions and adhesion molecule generation. Moreover, NF-κB activation was inhibited by myricetin via reducing IκB-α phosphorylation and p50/p65 subunit level. Notably, myricetin potentially attenuated vascular permeability of endothelial cells through decrease in eNOS phosphorylation and intracellular calcium elevation. These results indicate that myricetin from R. tomentosa possesses a promissing pharmaceutical property for the amelioration of endothelial inflammatory responses.

中文翻译：

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来自 Rodomyrtus tomentosa (Aiton) Hassk 果实的杨梅素减弱暴露于组胺的内皮细胞的炎症反应

摘要 组胺通过增加趋化因子和粘附分子的产生以及增加内皮细胞的血管通透性，在炎症反应中起关键作用。红桃已被发现是结构多样且具有生物活性代谢物的丰富来源。在这项研究中，研究了毛茸茸 (Aiton) Hassk 果实中的酚类化合物在下调组胺诱导的 EA.hy926 内皮细胞活化中的作用。在本文中，通过高效液相色谱法成功地从毛茸茸果实中分离出杨梅素，并通过质谱仪和核磁共振光谱对其进行了鉴定。结果表明，杨梅素可有效抑制 IL-8 和 MCP-1 的产生以及粘附分子的产生。而且，杨梅素通过降低 IκB-α 磷酸化和 p50/p65 亚基水平来抑制 NF-κB 活化。值得注意的是，杨梅素可能通过降低 eNOS 磷酸化和细胞内钙离子升高来减弱内皮细胞的血管通透性。这些结果表明，来自毛茸茸的杨梅素具有改善内皮炎症反应的有希望的药物特性。